Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12745 and BPHYT_RS12750 are separated by 122 nucleotides BPHYT_RS12750 and BPHYT_RS12755 are separated by 15 nucleotides BPHYT_RS12755 and BPHYT_RS12760 are separated by 154 nucleotides BPHYT_RS12760 and BPHYT_RS12765 are separated by 109 nucleotides BPHYT_RS12765 and BPHYT_RS12770 are separated by 164 nucleotides
BPHYT_RS12745: BPHYT_RS12745 - membrane protein, at 2,907,518 to 2,908,261
_RS12745
BPHYT_RS12750: BPHYT_RS12750 - hypothetical protein, at 2,908,384 to 2,908,581
_RS12750
BPHYT_RS12755: BPHYT_RS12755 - 2Fe-2S type ferredoxin, at 2,908,597 to 2,908,938
_RS12755
BPHYT_RS12760: BPHYT_RS12760 - molecular chaperone HscA, at 2,909,093 to 2,910,964
_RS12760
BPHYT_RS12765: BPHYT_RS12765 - Co-chaperone protein HscB homolog, at 2,911,074 to 2,911,601
_RS12765
BPHYT_RS12770: BPHYT_RS12770 - heme biosynthesis protein HemY, at 2,911,766 to 2,912,089
_RS12770
Position (kb)
2909
2910
2911 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2908.190 kb on + strand at 2908.190 kb on + strand at 2908.190 kb on + strand at 2908.191 kb on - strand at 2908.191 kb on - strand at 2908.191 kb on - strand at 2908.995 kb on - strand at 2909.017 kb on - strand at 2909.440 kb on - strand, within BPHYT_RS12760 at 2911.020 kb on - strand at 2911.020 kb on - strand at 2911.020 kb on - strand at 2911.076 kb on - strand at 2911.076 kb on - strand at 2911.076 kb on - strand at 2911.715 kb on - strand at 2911.768 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,908,190 + +1.4 2,908,190 + -2.5 2,908,190 + -1.1 2,908,191 - -0.4 2,908,191 - +1.4 2,908,191 - -0.1 2,908,995 - -0.0 2,909,017 - +3.5 2,909,440 - BPHYT_RS12760 0.19 +2.6 2,911,020 - +1.2 2,911,020 - +0.1 2,911,020 - +1.2 2,911,076 - -0.6 2,911,076 - -1.0 2,911,076 - +0.4 2,911,715 - +0.4 2,911,768 - -0.0
Or see this region's nucleotide sequence