Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12475 and BPHYT_RS12480 overlap by 14 nucleotides BPHYT_RS12480 and BPHYT_RS12485 overlap by 21 nucleotides BPHYT_RS12485 and BPHYT_RS12490 are separated by 32 nucleotides
BPHYT_RS12475: BPHYT_RS12475 - DNA mismatch repair protein MutS, at 2,843,553 to 2,846,237
_RS12475
BPHYT_RS12480: BPHYT_RS12480 - hypothetical protein, at 2,846,224 to 2,847,582
_RS12480
BPHYT_RS12485: BPHYT_RS12485 - peptidyl-prolyl cis-trans isomerase, at 2,847,562 to 2,848,104
_RS12485
BPHYT_RS12490: BPHYT_RS12490 - cupin, at 2,848,137 to 2,849,387
_RS12490
Position (kb)
2846
2847
2848 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 2845.509 kb on + strand, within BPHYT_RS12475 at 2845.510 kb on - strand, within BPHYT_RS12475 at 2845.521 kb on + strand, within BPHYT_RS12475 at 2845.521 kb on + strand, within BPHYT_RS12475 at 2845.522 kb on - strand, within BPHYT_RS12475 at 2845.773 kb on - strand, within BPHYT_RS12475 at 2845.777 kb on + strand, within BPHYT_RS12475 at 2845.777 kb on + strand, within BPHYT_RS12475 at 2846.365 kb on + strand, within BPHYT_RS12480 at 2846.383 kb on + strand at 2846.492 kb on - strand, within BPHYT_RS12480 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.695 kb on - strand, within BPHYT_RS12480 at 2846.834 kb on - strand, within BPHYT_RS12480 at 2847.222 kb on + strand, within BPHYT_RS12480 at 2847.896 kb on + strand, within BPHYT_RS12485 at 2847.897 kb on - strand, within BPHYT_RS12485 at 2847.995 kb on + strand, within BPHYT_RS12485 at 2847.996 kb on - strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.010 kb on + strand, within BPHYT_RS12485 at 2848.067 kb on + strand at 2848.068 kb on - strand at 2848.120 kb on - strand at 2848.132 kb on - strand at 2848.133 kb on + strand at 2848.133 kb on + strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.134 kb on - strand at 2848.421 kb on + strand, within BPHYT_RS12490 at 2848.422 kb on - strand, within BPHYT_RS12490
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 2,845,509 + BPHYT_RS12475 0.73 +4.2 2,845,510 - BPHYT_RS12475 0.73 +1.2 2,845,521 + BPHYT_RS12475 0.73 +0.2 2,845,521 + BPHYT_RS12475 0.73 +0.4 2,845,522 - BPHYT_RS12475 0.73 -1.0 2,845,773 - BPHYT_RS12475 0.83 -3.6 2,845,777 + BPHYT_RS12475 0.83 +1.7 2,845,777 + BPHYT_RS12475 0.83 -0.3 2,846,365 + BPHYT_RS12480 0.10 -2.1 2,846,383 + +1.0 2,846,492 - BPHYT_RS12480 0.20 -0.1 2,846,695 - BPHYT_RS12480 0.35 -0.8 2,846,695 - BPHYT_RS12480 0.35 +1.2 2,846,834 - BPHYT_RS12480 0.45 -1.7 2,847,222 + BPHYT_RS12480 0.73 +0.3 2,847,896 + BPHYT_RS12485 0.62 -1.1 2,847,897 - BPHYT_RS12485 0.62 -0.3 2,847,995 + BPHYT_RS12485 0.80 -0.1 2,847,996 - BPHYT_RS12485 0.80 -1.1 2,848,010 + BPHYT_RS12485 0.83 +0.1 2,848,010 + BPHYT_RS12485 0.83 +1.0 2,848,067 + -0.6 2,848,068 - -0.8 2,848,120 - -0.5 2,848,132 - -0.7 2,848,133 + +0.6 2,848,133 + -2.0 2,848,134 - -1.7 2,848,134 - -3.8 2,848,134 - -1.4 2,848,134 - -2.8 2,848,421 + BPHYT_RS12490 0.23 -0.8 2,848,422 - BPHYT_RS12490 0.23 +0.4
Or see this region's nucleotide sequence