Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09295

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09290 and BPHYT_RS09295 are separated by 121 nucleotidesBPHYT_RS09295 and BPHYT_RS09300 are separated by 46 nucleotidesBPHYT_RS09300 and BPHYT_RS09305 are separated by 87 nucleotides BPHYT_RS09290: BPHYT_RS09290 - helicase, at 2,104,724 to 2,108,206 _RS09290 BPHYT_RS09295: BPHYT_RS09295 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 2,108,328 to 2,109,044 _RS09295 BPHYT_RS09300: BPHYT_RS09300 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 2,109,091 to 2,109,570 _RS09300 BPHYT_RS09305: BPHYT_RS09305 - alkyl hydroperoxide reductase, at 2,109,658 to 2,110,206 _RS09305 Position (kb) 2108 2109 2110Strain fitness (log2 ratio) -1 0 1 2at 2107.398 kb on + strand, within BPHYT_RS09290at 2107.693 kb on - strand, within BPHYT_RS09290at 2107.801 kb on - strand, within BPHYT_RS09290at 2108.178 kb on + strandat 2108.206 kb on + strandat 2108.252 kb on - strandat 2108.282 kb on + strandat 2108.283 kb on - strandat 2108.288 kb on - strandat 2109.893 kb on + strand, within BPHYT_RS09305

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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2,107,398 + BPHYT_RS09290 0.77 +0.5
2,107,693 - BPHYT_RS09290 0.85 +0.1
2,107,801 - BPHYT_RS09290 0.88 -0.1
2,108,178 + +0.3
2,108,206 + +0.4
2,108,252 - -0.5
2,108,282 + -0.9
2,108,283 - +2.4
2,108,288 - +0.5
2,109,893 + BPHYT_RS09305 0.43 +0.8

Or see this region's nucleotide sequence