Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08685 and BPHYT_RS08690 are separated by 90 nucleotides BPHYT_RS08690 and BPHYT_RS08695 are separated by 157 nucleotides BPHYT_RS08695 and BPHYT_RS08700 are separated by 88 nucleotides
BPHYT_RS08685: BPHYT_RS08685 - hypothetical protein, at 1,974,732 to 1,974,956
_RS08685
BPHYT_RS08690: BPHYT_RS08690 - hypothetical protein, at 1,975,047 to 1,975,292
_RS08690
BPHYT_RS08695: BPHYT_RS08695 - tRNA-His, at 1,975,450 to 1,975,525
_RS08695
BPHYT_RS08700: BPHYT_RS08700 - tRNA-dihydrouridine synthase A, at 1,975,614 to 1,976,615
_RS08700
Position (kb) 1975
1976 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1974.507 kb on + strand at 1974.507 kb on + strand at 1974.519 kb on - strand at 1974.548 kb on + strand at 1974.677 kb on + strand at 1974.822 kb on - strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.861 kb on + strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.862 kb on - strand, within BPHYT_RS08685 at 1974.925 kb on + strand, within BPHYT_RS08685 at 1974.926 kb on - strand, within BPHYT_RS08685 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.104 kb on + strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.105 kb on - strand, within BPHYT_RS08690 at 1975.435 kb on - strand at 1975.698 kb on - strand at 1975.744 kb on + strand, within BPHYT_RS08700 at 1975.780 kb on + strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.781 kb on - strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1975.867 kb on + strand, within BPHYT_RS08700 at 1976.036 kb on - strand, within BPHYT_RS08700 at 1976.170 kb on + strand, within BPHYT_RS08700 at 1976.171 kb on - strand, within BPHYT_RS08700 at 1976.347 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700 at 1976.412 kb on + strand, within BPHYT_RS08700
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,974,507 + -0.5 1,974,507 + +0.5 1,974,519 - +0.4 1,974,548 + -0.2 1,974,677 + -0.2 1,974,822 - BPHYT_RS08685 0.40 +1.0 1,974,861 + BPHYT_RS08685 0.57 -0.0 1,974,861 + BPHYT_RS08685 0.57 -0.0 1,974,862 - BPHYT_RS08685 0.58 +1.7 1,974,862 - BPHYT_RS08685 0.58 +2.7 1,974,925 + BPHYT_RS08685 0.86 +0.5 1,974,926 - BPHYT_RS08685 0.86 +0.9 1,975,104 + BPHYT_RS08690 0.23 -0.0 1,975,104 + BPHYT_RS08690 0.23 -0.9 1,975,104 + BPHYT_RS08690 0.23 -2.0 1,975,104 + BPHYT_RS08690 0.23 +0.2 1,975,105 - BPHYT_RS08690 0.24 +0.2 1,975,105 - BPHYT_RS08690 0.24 -1.9 1,975,435 - -0.5 1,975,698 - +1.1 1,975,744 + BPHYT_RS08700 0.13 -0.0 1,975,780 + BPHYT_RS08700 0.17 -0.8 1,975,781 - BPHYT_RS08700 0.17 -0.4 1,975,781 - BPHYT_RS08700 0.17 -2.7 1,975,867 + BPHYT_RS08700 0.25 -1.3 1,975,867 + BPHYT_RS08700 0.25 -0.9 1,976,036 - BPHYT_RS08700 0.42 +1.6 1,976,170 + BPHYT_RS08700 0.55 -2.2 1,976,171 - BPHYT_RS08700 0.56 -1.1 1,976,347 + BPHYT_RS08700 0.73 -0.1 1,976,412 + BPHYT_RS08700 0.80 -0.3 1,976,412 + BPHYT_RS08700 0.80 -1.3
Or see this region's nucleotide sequence