Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS07250

Experiment: 24hr PHYTO3 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS07240 and BPHYT_RS07245 are separated by 11 nucleotidesBPHYT_RS07245 and BPHYT_RS07250 are separated by 13 nucleotidesBPHYT_RS07250 and BPHYT_RS07255 overlap by 4 nucleotidesBPHYT_RS07255 and BPHYT_RS07260 overlap by 4 nucleotides BPHYT_RS07240: BPHYT_RS07240 - 2-keto-4-pentenoate hydratase, at 1,644,639 to 1,645,460 _RS07240 BPHYT_RS07245: BPHYT_RS07245 - acetaldehyde dehydrogenase, at 1,645,472 to 1,646,416 _RS07245 BPHYT_RS07250: BPHYT_RS07250 - 4-hydroxy-2-oxovalerate aldolase, at 1,646,430 to 1,647,473 _RS07250 BPHYT_RS07255: BPHYT_RS07255 - 4-oxalocrotonate decarboxylase, at 1,647,470 to 1,648,243 _RS07255 BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671 _RS07260 Position (kb) 1646 1647 1648Strain fitness (log2 ratio) -2 -1 0 1 2at 1646.336 kb on + strandat 1646.565 kb on - strand, within BPHYT_RS07250at 1646.858 kb on + strand, within BPHYT_RS07250at 1646.926 kb on - strand, within BPHYT_RS07250at 1647.339 kb on + strand, within BPHYT_RS07250at 1647.650 kb on - strand, within BPHYT_RS07255at 1647.669 kb on + strand, within BPHYT_RS07255at 1647.669 kb on + strand, within BPHYT_RS07255at 1647.670 kb on - strand, within BPHYT_RS07255at 1648.230 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10
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1,646,336 + -0.8
1,646,565 - BPHYT_RS07250 0.13 -0.8
1,646,858 + BPHYT_RS07250 0.41 -0.8
1,646,926 - BPHYT_RS07250 0.48 +0.7
1,647,339 + BPHYT_RS07250 0.87 -0.5
1,647,650 - BPHYT_RS07255 0.23 -2.0
1,647,669 + BPHYT_RS07255 0.26 +2.0
1,647,669 + BPHYT_RS07255 0.26 -0.7
1,647,670 - BPHYT_RS07255 0.26 +0.5
1,648,230 - -0.3

Or see this region's nucleotide sequence