Experiment: 24hr PHYTO3 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07240 and BPHYT_RS07245 are separated by 11 nucleotides BPHYT_RS07245 and BPHYT_RS07250 are separated by 13 nucleotides BPHYT_RS07250 and BPHYT_RS07255 overlap by 4 nucleotides BPHYT_RS07255 and BPHYT_RS07260 overlap by 4 nucleotides
BPHYT_RS07240: BPHYT_RS07240 - 2-keto-4-pentenoate hydratase, at 1,644,639 to 1,645,460
_RS07240
BPHYT_RS07245: BPHYT_RS07245 - acetaldehyde dehydrogenase, at 1,645,472 to 1,646,416
_RS07245
BPHYT_RS07250: BPHYT_RS07250 - 4-hydroxy-2-oxovalerate aldolase, at 1,646,430 to 1,647,473
_RS07250
BPHYT_RS07255: BPHYT_RS07255 - 4-oxalocrotonate decarboxylase, at 1,647,470 to 1,648,243
_RS07255
BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671
_RS07260
Position (kb)
1646
1647
1648 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1646.336 kb on + strand at 1646.565 kb on - strand, within BPHYT_RS07250 at 1646.858 kb on + strand, within BPHYT_RS07250 at 1646.926 kb on - strand, within BPHYT_RS07250 at 1647.339 kb on + strand, within BPHYT_RS07250 at 1647.650 kb on - strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.670 kb on - strand, within BPHYT_RS07255 at 1648.230 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO3 MOI 10 remove 1,646,336 + -0.8 1,646,565 - BPHYT_RS07250 0.13 -0.8 1,646,858 + BPHYT_RS07250 0.41 -0.8 1,646,926 - BPHYT_RS07250 0.48 +0.7 1,647,339 + BPHYT_RS07250 0.87 -0.5 1,647,650 - BPHYT_RS07255 0.23 -2.0 1,647,669 + BPHYT_RS07255 0.26 +2.0 1,647,669 + BPHYT_RS07255 0.26 -0.7 1,647,670 - BPHYT_RS07255 0.26 +0.5 1,648,230 - -0.3
Or see this region's nucleotide sequence