Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31170 and BPHYT_RS31175 are separated by 145 nucleotides
BPHYT_RS31170: BPHYT_RS31170 - hypothetical protein, at 2,550,548 to 2,552,365
_RS31170
BPHYT_RS31175: BPHYT_RS31175 - RNA polymerase sigma70 factor, at 2,552,511 to 2,553,785
_RS31175
Position (kb)
2550
2551
2552
2553 Strain fitness (log2 ratio)
-1
0
1
2 at 2549.611 kb on - strand at 2549.691 kb on - strand at 2550.196 kb on - strand at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.049 kb on + strand, within BPHYT_RS31170 at 2551.235 kb on + strand, within BPHYT_RS31170 at 2551.235 kb on + strand, within BPHYT_RS31170 at 2551.236 kb on - strand, within BPHYT_RS31170 at 2551.238 kb on - strand, within BPHYT_RS31170 at 2551.537 kb on - strand, within BPHYT_RS31170 at 2551.625 kb on - strand, within BPHYT_RS31170 at 2551.762 kb on + strand, within BPHYT_RS31170 at 2551.763 kb on - strand, within BPHYT_RS31170 at 2551.770 kb on + strand, within BPHYT_RS31170 at 2552.057 kb on - strand, within BPHYT_RS31170 at 2552.453 kb on + strand at 2552.453 kb on + strand at 2552.454 kb on - strand at 2552.454 kb on - strand at 2552.713 kb on + strand, within BPHYT_RS31175 at 2552.871 kb on + strand, within BPHYT_RS31175 at 2552.872 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2552.982 kb on - strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.010 kb on + strand, within BPHYT_RS31175 at 2553.011 kb on - strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175 at 2553.055 kb on + strand, within BPHYT_RS31175
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 2,549,611 - -0.5 2,549,691 - +0.3 2,550,196 - +0.2 2,551,049 + BPHYT_RS31170 0.28 -0.2 2,551,049 + BPHYT_RS31170 0.28 -0.3 2,551,049 + BPHYT_RS31170 0.28 -0.6 2,551,235 + BPHYT_RS31170 0.38 -0.1 2,551,235 + BPHYT_RS31170 0.38 -0.7 2,551,236 - BPHYT_RS31170 0.38 +0.1 2,551,238 - BPHYT_RS31170 0.38 -0.4 2,551,537 - BPHYT_RS31170 0.54 -0.5 2,551,625 - BPHYT_RS31170 0.59 +0.3 2,551,762 + BPHYT_RS31170 0.67 +0.6 2,551,763 - BPHYT_RS31170 0.67 -0.6 2,551,770 + BPHYT_RS31170 0.67 +0.3 2,552,057 - BPHYT_RS31170 0.83 -0.9 2,552,453 + -0.4 2,552,453 + +0.2 2,552,454 - -0.5 2,552,454 - +0.4 2,552,713 + BPHYT_RS31175 0.16 -0.3 2,552,871 + BPHYT_RS31175 0.28 +2.0 2,552,872 - BPHYT_RS31175 0.28 -0.8 2,552,982 - BPHYT_RS31175 0.37 -0.9 2,552,982 - BPHYT_RS31175 0.37 +0.4 2,552,982 - BPHYT_RS31175 0.37 +1.4 2,553,010 + BPHYT_RS31175 0.39 +0.4 2,553,010 + BPHYT_RS31175 0.39 +0.8 2,553,011 - BPHYT_RS31175 0.39 -0.1 2,553,055 + BPHYT_RS31175 0.43 +0.3 2,553,055 + BPHYT_RS31175 0.43 +0.7
Or see this region's nucleotide sequence