Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16110 and BPHYT_RS16115 are separated by 152 nucleotides BPHYT_RS16115 and BPHYT_RS16120 are separated by 72 nucleotides
BPHYT_RS16110: BPHYT_RS16110 - ABC transporter for D-Sorbitol, permease component 2 (from data) , at 3,654,806 to 3,655,741
_RS16110
BPHYT_RS16115: BPHYT_RS16115 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data) , at 3,655,894 to 3,657,216
_RS16115
BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data) , at 3,657,289 to 3,658,071
_RS16120
Position (kb)
3655
3656
3657
3658 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3654.942 kb on + strand, within BPHYT_RS16110 at 3654.942 kb on + strand, within BPHYT_RS16110 at 3655.415 kb on + strand, within BPHYT_RS16110 at 3655.518 kb on + strand, within BPHYT_RS16110 at 3655.523 kb on - strand, within BPHYT_RS16110 at 3655.523 kb on - strand, within BPHYT_RS16110 at 3655.572 kb on + strand, within BPHYT_RS16110 at 3655.720 kb on + strand at 3655.720 kb on + strand at 3655.720 kb on + strand at 3655.721 kb on - strand at 3656.147 kb on + strand, within BPHYT_RS16115 at 3656.229 kb on - strand, within BPHYT_RS16115 at 3656.400 kb on - strand, within BPHYT_RS16115 at 3656.515 kb on + strand, within BPHYT_RS16115 at 3656.528 kb on + strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.529 kb on - strand, within BPHYT_RS16115 at 3656.627 kb on + strand at 3656.675 kb on + strand, within BPHYT_RS16115 at 3656.730 kb on - strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.786 kb on + strand, within BPHYT_RS16115 at 3656.787 kb on - strand, within BPHYT_RS16115 at 3656.807 kb on + strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.808 kb on - strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3656.822 kb on + strand, within BPHYT_RS16115 at 3657.240 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.244 kb on + strand at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.461 kb on + strand, within BPHYT_RS16120 at 3657.501 kb on + strand, within BPHYT_RS16120 at 3657.576 kb on - strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.651 kb on + strand, within BPHYT_RS16120 at 3657.652 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.915 kb on - strand, within BPHYT_RS16120 at 3657.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 3,654,942 + BPHYT_RS16110 0.15 -0.4 3,654,942 + BPHYT_RS16110 0.15 -1.0 3,655,415 + BPHYT_RS16110 0.65 -0.6 3,655,518 + BPHYT_RS16110 0.76 -0.2 3,655,523 - BPHYT_RS16110 0.77 -0.0 3,655,523 - BPHYT_RS16110 0.77 -0.4 3,655,572 + BPHYT_RS16110 0.82 -0.7 3,655,720 + -0.9 3,655,720 + +0.6 3,655,720 + -1.4 3,655,721 - +1.2 3,656,147 + BPHYT_RS16115 0.19 +1.1 3,656,229 - BPHYT_RS16115 0.25 -0.4 3,656,400 - BPHYT_RS16115 0.38 +1.1 3,656,515 + BPHYT_RS16115 0.47 -1.1 3,656,528 + BPHYT_RS16115 0.48 +0.1 3,656,529 - BPHYT_RS16115 0.48 -1.9 3,656,529 - BPHYT_RS16115 0.48 -0.8 3,656,627 + -1.1 3,656,675 + BPHYT_RS16115 0.59 +0.3 3,656,730 - BPHYT_RS16115 0.63 -2.1 3,656,786 + BPHYT_RS16115 0.67 -1.2 3,656,786 + BPHYT_RS16115 0.67 +1.3 3,656,786 + BPHYT_RS16115 0.67 -0.4 3,656,787 - BPHYT_RS16115 0.67 +3.2 3,656,807 + BPHYT_RS16115 0.69 -1.4 3,656,808 - BPHYT_RS16115 0.69 -0.5 3,656,808 - BPHYT_RS16115 0.69 +0.2 3,656,822 + BPHYT_RS16115 0.70 -1.8 3,656,822 + BPHYT_RS16115 0.70 +0.5 3,657,240 + +0.9 3,657,244 + +1.0 3,657,244 + -0.1 3,657,244 + -0.4 3,657,461 + BPHYT_RS16120 0.22 +1.8 3,657,461 + BPHYT_RS16120 0.22 +0.4 3,657,501 + BPHYT_RS16120 0.27 +1.0 3,657,576 - BPHYT_RS16120 0.37 -0.8 3,657,651 + BPHYT_RS16120 0.46 -1.0 3,657,651 + BPHYT_RS16120 0.46 +3.3 3,657,652 - BPHYT_RS16120 0.46 +2.2 3,657,915 - BPHYT_RS16120 0.80 -2.3 3,657,915 - BPHYT_RS16120 0.80 -0.1 3,657,993 - +0.6
Or see this region's nucleotide sequence