Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS12255

Experiment: 24hr PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS12250 and BPHYT_RS12255 are separated by 246 nucleotidesBPHYT_RS12255 and BPHYT_RS12260 overlap by 4 nucleotides BPHYT_RS12250: BPHYT_RS12250 - phosphomethylpyrimidine kinase, at 2,798,094 to 2,798,900 _RS12250 BPHYT_RS12255: BPHYT_RS12255 - hypothetical protein, at 2,799,147 to 2,800,160 _RS12255 BPHYT_RS12260: BPHYT_RS12260 - DNA polymerase, at 2,800,157 to 2,801,401 _RS12260 Position (kb) 2799 2800 2801Strain fitness (log2 ratio) -1 0 1 2 3at 2798.917 kb on + strandat 2798.917 kb on + strandat 2798.918 kb on - strandat 2799.018 kb on + strandat 2799.197 kb on + strandat 2799.198 kb on - strandat 2799.198 kb on - strandat 2799.219 kb on + strandat 2799.220 kb on - strandat 2799.220 kb on - strandat 2799.344 kb on + strand, within BPHYT_RS12255at 2799.344 kb on + strand, within BPHYT_RS12255at 2799.344 kb on + strand, within BPHYT_RS12255at 2799.344 kb on + strand, within BPHYT_RS12255at 2799.345 kb on - strand, within BPHYT_RS12255at 2799.345 kb on - strand, within BPHYT_RS12255at 2799.345 kb on - strand, within BPHYT_RS12255at 2799.345 kb on - strand, within BPHYT_RS12255at 2799.345 kb on - strand, within BPHYT_RS12255at 2799.652 kb on + strand, within BPHYT_RS12255at 2799.673 kb on - strand, within BPHYT_RS12255at 2799.703 kb on + strand, within BPHYT_RS12255at 2799.703 kb on + strand, within BPHYT_RS12255at 2799.703 kb on + strand, within BPHYT_RS12255at 2799.703 kb on + strand, within BPHYT_RS12255at 2799.703 kb on + strand, within BPHYT_RS12255at 2800.080 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1
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2,798,917 + -0.2
2,798,917 + -0.9
2,798,918 - +1.4
2,799,018 + +0.0
2,799,197 + -0.7
2,799,198 - -0.7
2,799,198 - -0.4
2,799,219 + -0.6
2,799,220 - -0.6
2,799,220 - -0.5
2,799,344 + BPHYT_RS12255 0.19 +1.0
2,799,344 + BPHYT_RS12255 0.19 +0.2
2,799,344 + BPHYT_RS12255 0.19 -0.9
2,799,344 + BPHYT_RS12255 0.19 -0.7
2,799,345 - BPHYT_RS12255 0.20 -0.9
2,799,345 - BPHYT_RS12255 0.20 +0.6
2,799,345 - BPHYT_RS12255 0.20 -0.3
2,799,345 - BPHYT_RS12255 0.20 +0.6
2,799,345 - BPHYT_RS12255 0.20 -0.4
2,799,652 + BPHYT_RS12255 0.50 +0.5
2,799,673 - BPHYT_RS12255 0.52 -0.5
2,799,703 + BPHYT_RS12255 0.55 -0.6
2,799,703 + BPHYT_RS12255 0.55 +0.2
2,799,703 + BPHYT_RS12255 0.55 +0.1
2,799,703 + BPHYT_RS12255 0.55 +3.6
2,799,703 + BPHYT_RS12255 0.55 -0.3
2,800,080 - +0.6

Or see this region's nucleotide sequence