Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35215 and BPHYT_RS35220 are separated by 186 nucleotides BPHYT_RS35220 and BPHYT_RS35225 are separated by 4 nucleotides BPHYT_RS35225 and BPHYT_RS35230 are separated by 36 nucleotides BPHYT_RS35230 and BPHYT_RS35235 are separated by 183 nucleotides
BPHYT_RS35215: BPHYT_RS35215 - dehydrogenase, at 3,505,507 to 3,505,920
_RS35215
BPHYT_RS35220: BPHYT_RS35220 - extradiol dioxygenase, at 3,506,107 to 3,506,514
_RS35220
BPHYT_RS35225: BPHYT_RS35225 - RNA polymerase subunit sigma-24, at 3,506,519 to 3,507,793
_RS35225
BPHYT_RS35230: BPHYT_RS35230 - type 11 methyltransferase, at 3,507,830 to 3,508,519
_RS35230
BPHYT_RS35235: BPHYT_RS35235 - amino acid transporter, at 3,508,703 to 3,509,320
_RS35235
Position (kb)
3506
3507
3508 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3505.743 kb on + strand, within BPHYT_RS35215 at 3506.113 kb on - strand at 3506.123 kb on - strand at 3506.246 kb on - strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.434 kb on + strand, within BPHYT_RS35220 at 3506.435 kb on - strand, within BPHYT_RS35220 at 3506.435 kb on - strand, within BPHYT_RS35220 at 3506.482 kb on + strand at 3506.483 kb on - strand at 3506.861 kb on - strand, within BPHYT_RS35225 at 3507.119 kb on + strand, within BPHYT_RS35225 at 3507.141 kb on - strand, within BPHYT_RS35225 at 3507.641 kb on + strand, within BPHYT_RS35225 at 3507.641 kb on + strand, within BPHYT_RS35225 at 3507.661 kb on - strand, within BPHYT_RS35225 at 3507.987 kb on + strand, within BPHYT_RS35230 at 3508.253 kb on - strand, within BPHYT_RS35230 at 3508.253 kb on - strand at 3508.579 kb on + strand at 3508.628 kb on - strand at 3508.698 kb on - strand at 3508.783 kb on - strand, within BPHYT_RS35235
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 3,505,743 + BPHYT_RS35215 0.57 +0.6 3,506,113 - -0.8 3,506,123 - -2.1 3,506,246 - BPHYT_RS35220 0.34 -0.1 3,506,434 + BPHYT_RS35220 0.80 +0.1 3,506,434 + BPHYT_RS35220 0.80 -0.7 3,506,434 + BPHYT_RS35220 0.80 +1.4 3,506,435 - BPHYT_RS35220 0.80 +0.1 3,506,435 - BPHYT_RS35220 0.80 +1.8 3,506,482 + -0.9 3,506,483 - -1.0 3,506,861 - BPHYT_RS35225 0.27 +0.5 3,507,119 + BPHYT_RS35225 0.47 +0.5 3,507,141 - BPHYT_RS35225 0.49 -0.6 3,507,641 + BPHYT_RS35225 0.88 +0.2 3,507,641 + BPHYT_RS35225 0.88 +0.4 3,507,661 - BPHYT_RS35225 0.90 -0.2 3,507,987 + BPHYT_RS35230 0.23 -0.7 3,508,253 - BPHYT_RS35230 0.61 -0.8 3,508,253 - +1.4 3,508,579 + +0.9 3,508,628 - +0.2 3,508,698 - +0.7 3,508,783 - BPHYT_RS35235 0.13 -1.9
Or see this region's nucleotide sequence