Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25875 and BPHYT_RS25880 are separated by 1 nucleotides BPHYT_RS25880 and BPHYT_RS25885 are separated by 32 nucleotides BPHYT_RS25885 and BPHYT_RS25890 overlap by 1 nucleotides BPHYT_RS25890 and BPHYT_RS25895 overlap by 4 nucleotides
BPHYT_RS25875: BPHYT_RS25875 - type III secretion protein, at 1,384,937 to 1,387,012
_RS25875
BPHYT_RS25880: BPHYT_RS25880 - type III secretion protein, at 1,387,014 to 1,387,754
_RS25880
BPHYT_RS25885: BPHYT_RS25885 - type III secretion protein, at 1,387,787 to 1,388,476
_RS25885
BPHYT_RS25890: BPHYT_RS25890 - type III secretion protein, at 1,388,476 to 1,388,751
_RS25890
BPHYT_RS25895: BPHYT_RS25895 - type III secretion system protein, at 1,388,748 to 1,389,395
_RS25895
Position (kb) 1387
1388 Strain fitness (log2 ratio) -1
0
1 at 1386.304 kb on - strand, within BPHYT_RS25875 at 1386.445 kb on - strand, within BPHYT_RS25875 at 1386.885 kb on - strand at 1387.760 kb on + strand at 1387.760 kb on + strand at 1388.173 kb on - strand, within BPHYT_RS25885 at 1388.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 1,386,304 - BPHYT_RS25875 0.66 -0.9 1,386,445 - BPHYT_RS25875 0.73 -0.6 1,386,885 - -0.1 1,387,760 + -1.1 1,387,760 + -1.4 1,388,173 - BPHYT_RS25885 0.56 -0.8 1,388,463 - +0.1
Or see this region's nucleotide sequence