Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24095 and BPHYT_RS24100 are separated by 207 nucleotides BPHYT_RS24100 and BPHYT_RS24105 are separated by 298 nucleotides BPHYT_RS24105 and BPHYT_RS24110 are separated by 110 nucleotides
BPHYT_RS24095: BPHYT_RS24095 - membrane protein, at 972,419 to 973,831
_RS24095
BPHYT_RS24100: BPHYT_RS24100 - hypothetical protein, at 974,039 to 974,299
_RS24100
BPHYT_RS24105: BPHYT_RS24105 - chloroperoxidase, at 974,598 to 975,419
_RS24105
BPHYT_RS24110: BPHYT_RS24110 - hypothetical protein, at 975,530 to 976,072
_RS24110
Position (kb) 974
975
976 Strain fitness (log2 ratio) -1
0
1
2
3 at 973.680 kb on + strand, within BPHYT_RS24095 at 973.681 kb on - strand, within BPHYT_RS24095 at 973.683 kb on + strand, within BPHYT_RS24095 at 973.684 kb on - strand, within BPHYT_RS24095 at 974.298 kb on - strand at 974.314 kb on - strand at 974.394 kb on - strand at 974.582 kb on + strand at 974.582 kb on + strand at 974.582 kb on + strand at 974.814 kb on + strand, within BPHYT_RS24105 at 975.177 kb on + strand, within BPHYT_RS24105 at 975.616 kb on - strand, within BPHYT_RS24110 at 975.666 kb on + strand, within BPHYT_RS24110 at 975.966 kb on - strand, within BPHYT_RS24110 at 975.978 kb on + strand, within BPHYT_RS24110 at 976.088 kb on + strand at 976.089 kb on - strand at 976.232 kb on - strand at 976.326 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 973,680 + BPHYT_RS24095 0.89 +2.3 973,681 - BPHYT_RS24095 0.89 +1.0 973,683 + BPHYT_RS24095 0.89 +2.3 973,684 - BPHYT_RS24095 0.90 +0.8 974,298 - -0.4 974,314 - -0.1 974,394 - -0.8 974,582 + +0.1 974,582 + +3.2 974,582 + +1.0 974,814 + BPHYT_RS24105 0.26 +0.6 975,177 + BPHYT_RS24105 0.70 +0.4 975,616 - BPHYT_RS24110 0.16 +0.2 975,666 + BPHYT_RS24110 0.25 -1.6 975,966 - BPHYT_RS24110 0.80 -0.8 975,978 + BPHYT_RS24110 0.83 +2.8 976,088 + -0.8 976,089 - +0.2 976,232 - +0.3 976,326 + +0.6
Or see this region's nucleotide sequence