Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22465 and BPHYT_RS22470 overlap by 4 nucleotides BPHYT_RS22470 and BPHYT_RS22475 are separated by 169 nucleotides BPHYT_RS22475 and BPHYT_RS22480 are separated by 197 nucleotides
BPHYT_RS22465: BPHYT_RS22465 - zinc carboxypeptidase-like protein, at 595,452 to 596,486
_RS22465
BPHYT_RS22470: BPHYT_RS22470 - hydrolase, at 596,483 to 597,283
_RS22470
BPHYT_RS22475: BPHYT_RS22475 - NAD-glutamate dehydrogenase, at 597,453 to 602,294
_RS22475
BPHYT_RS22480: BPHYT_RS22480 - cytochrome C551, at 602,492 to 603,397
_RS22480
Position (kb) 597
598
599
600
601
602
603 Strain fitness (log2 ratio) -2
-1
0
1 at 596.467 kb on - strand at 596.468 kb on + strand at 596.482 kb on + strand at 596.816 kb on - strand, within BPHYT_RS22470 at 597.325 kb on + strand at 597.371 kb on + strand at 597.371 kb on + strand at 597.372 kb on - strand at 597.622 kb on - strand at 597.691 kb on - strand at 597.691 kb on - strand at 597.826 kb on + strand at 598.624 kb on - strand, within BPHYT_RS22475 at 598.624 kb on - strand, within BPHYT_RS22475 at 599.044 kb on - strand, within BPHYT_RS22475 at 599.631 kb on - strand at 599.680 kb on + strand, within BPHYT_RS22475 at 599.727 kb on + strand, within BPHYT_RS22475 at 599.727 kb on + strand, within BPHYT_RS22475 at 599.764 kb on - strand, within BPHYT_RS22475 at 600.172 kb on - strand, within BPHYT_RS22475 at 600.452 kb on + strand, within BPHYT_RS22475 at 600.736 kb on - strand, within BPHYT_RS22475 at 601.093 kb on - strand, within BPHYT_RS22475 at 601.096 kb on - strand, within BPHYT_RS22475 at 601.096 kb on - strand, within BPHYT_RS22475 at 601.311 kb on + strand, within BPHYT_RS22475 at 602.196 kb on + strand at 602.292 kb on + strand at 602.777 kb on + strand, within BPHYT_RS22480 at 602.778 kb on - strand, within BPHYT_RS22480 at 602.819 kb on + strand, within BPHYT_RS22480 at 603.229 kb on + strand, within BPHYT_RS22480
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 596,467 - +0.1 596,468 + -1.5 596,482 + -0.3 596,816 - BPHYT_RS22470 0.42 -1.0 597,325 + +1.6 597,371 + +1.1 597,371 + -0.6 597,372 - +0.2 597,622 - -0.8 597,691 - +0.3 597,691 - +0.8 597,826 + +0.6 598,624 - BPHYT_RS22475 0.24 +0.1 598,624 - BPHYT_RS22475 0.24 +1.7 599,044 - BPHYT_RS22475 0.33 +0.7 599,631 - -0.1 599,680 + BPHYT_RS22475 0.46 -1.9 599,727 + BPHYT_RS22475 0.47 +1.0 599,727 + BPHYT_RS22475 0.47 -1.6 599,764 - BPHYT_RS22475 0.48 -0.1 600,172 - BPHYT_RS22475 0.56 +0.0 600,452 + BPHYT_RS22475 0.62 -1.1 600,736 - BPHYT_RS22475 0.68 -0.0 601,093 - BPHYT_RS22475 0.75 +0.1 601,096 - BPHYT_RS22475 0.75 +1.2 601,096 - BPHYT_RS22475 0.75 +0.2 601,311 + BPHYT_RS22475 0.80 +0.2 602,196 + -0.4 602,292 + -1.2 602,777 + BPHYT_RS22480 0.31 -0.1 602,778 - BPHYT_RS22480 0.32 -0.2 602,819 + BPHYT_RS22480 0.36 +0.5 603,229 + BPHYT_RS22480 0.81 +0.3
Or see this region's nucleotide sequence