Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13325 and BPHYT_RS13330 overlap by 23 nucleotides BPHYT_RS13330 and BPHYT_RS13335 are separated by 56 nucleotides BPHYT_RS13335 and BPHYT_RS13340 are separated by 60 nucleotides
BPHYT_RS13325: BPHYT_RS13325 - TetR family transcriptional regulator, at 3,043,322 to 3,044,032
_RS13325
BPHYT_RS13330: BPHYT_RS13330 - prevent-host-death protein, at 3,044,010 to 3,044,240
_RS13330
BPHYT_RS13335: BPHYT_RS13335 - benzoyl-CoA oxygenase, at 3,044,297 to 3,045,538
_RS13335
BPHYT_RS13340: BPHYT_RS13340 - benzoyl-CoA oxygenase, at 3,045,599 to 3,047,026
_RS13340
Position (kb) 3044
3045
3046 Strain fitness (log2 ratio) -1
0
1 at 3043.850 kb on - strand, within BPHYT_RS13325 at 3043.875 kb on - strand, within BPHYT_RS13325 at 3044.437 kb on + strand, within BPHYT_RS13335 at 3045.414 kb on + strand at 3045.467 kb on - strand at 3045.615 kb on + strand at 3045.931 kb on - strand, within BPHYT_RS13340 at 3046.018 kb on - strand, within BPHYT_RS13340 at 3046.018 kb on - strand, within BPHYT_RS13340 at 3046.087 kb on + strand, within BPHYT_RS13340 at 3046.090 kb on - strand, within BPHYT_RS13340
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 3,043,850 - BPHYT_RS13325 0.74 +0.1 3,043,875 - BPHYT_RS13325 0.78 -0.0 3,044,437 + BPHYT_RS13335 0.11 +1.2 3,045,414 + -0.5 3,045,467 - -0.1 3,045,615 + +1.4 3,045,931 - BPHYT_RS13340 0.23 -0.4 3,046,018 - BPHYT_RS13340 0.29 +0.2 3,046,018 - BPHYT_RS13340 0.29 -0.4 3,046,087 + BPHYT_RS13340 0.34 +0.2 3,046,090 - BPHYT_RS13340 0.34 +0.1
Or see this region's nucleotide sequence