Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10810

Experiment: 24hr PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10805 and BPHYT_RS10810 are separated by 93 nucleotidesBPHYT_RS10810 and BPHYT_RS10815 are separated by 45 nucleotidesBPHYT_RS10815 and BPHYT_RS10820 are separated by 3 nucleotides BPHYT_RS10805: BPHYT_RS10805 - MFS transporter, at 2,455,585 to 2,456,961 _RS10805 BPHYT_RS10810: BPHYT_RS10810 - glucose-methanol-choline oxidoreductase, at 2,457,055 to 2,458,788 _RS10810 BPHYT_RS10815: BPHYT_RS10815 - crotonase, at 2,458,834 to 2,459,640 _RS10815 BPHYT_RS10820: BPHYT_RS10820 - 4-chlorobenzoate--CoA ligase, at 2,459,644 to 2,461,221 _RS10820 Position (kb) 2457 2458 2459Strain fitness (log2 ratio) -1 0 1at 2456.130 kb on - strand, within BPHYT_RS10805at 2456.423 kb on + strand, within BPHYT_RS10805at 2456.496 kb on - strand, within BPHYT_RS10805at 2456.768 kb on + strand, within BPHYT_RS10805at 2456.768 kb on + strand, within BPHYT_RS10805at 2457.047 kb on - strandat 2457.219 kb on - strandat 2457.767 kb on + strand, within BPHYT_RS10810at 2457.768 kb on - strand, within BPHYT_RS10810at 2458.325 kb on + strand, within BPHYT_RS10810at 2458.325 kb on + strand, within BPHYT_RS10810at 2458.507 kb on + strand, within BPHYT_RS10810at 2458.773 kb on - strandat 2458.804 kb on - strandat 2458.804 kb on - strandat 2458.919 kb on + strand, within BPHYT_RS10815at 2458.919 kb on + strand, within BPHYT_RS10815at 2459.756 kb on - strandat 2459.774 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1
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2,456,130 - BPHYT_RS10805 0.40 +0.4
2,456,423 + BPHYT_RS10805 0.61 -1.3
2,456,496 - BPHYT_RS10805 0.66 -0.5
2,456,768 + BPHYT_RS10805 0.86 +1.4
2,456,768 + BPHYT_RS10805 0.86 +1.6
2,457,047 - -0.7
2,457,219 - -1.1
2,457,767 + BPHYT_RS10810 0.41 +0.2
2,457,768 - BPHYT_RS10810 0.41 -0.3
2,458,325 + BPHYT_RS10810 0.73 +0.6
2,458,325 + BPHYT_RS10810 0.73 +0.8
2,458,507 + BPHYT_RS10810 0.84 +0.8
2,458,773 - -0.5
2,458,804 - -0.6
2,458,804 - -0.8
2,458,919 + BPHYT_RS10815 0.11 -0.1
2,458,919 + BPHYT_RS10815 0.11 +1.7
2,459,756 - +0.3
2,459,774 + -0.4

Or see this region's nucleotide sequence