Experiment: 24hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01720 and BPHYT_RS01725 are separated by 427 nucleotides BPHYT_RS01725 and BPHYT_RS01730 are separated by 115 nucleotides BPHYT_RS01730 and BPHYT_RS01735 are separated by 27 nucleotides
BPHYT_RS01720: BPHYT_RS01720 - acriflavin resistance protein, at 389,373 to 392,555
_RS01720
BPHYT_RS01725: BPHYT_RS01725 - hypothetical protein, at 392,983 to 393,195
_RS01725
BPHYT_RS01730: BPHYT_RS01730 - serine/threonine protein kinase, at 393,311 to 394,339
_RS01730
BPHYT_RS01735: BPHYT_RS01735 - membrane protein, at 394,367 to 396,268
_RS01735
Position (kb) 393
394
395 Strain fitness (log2 ratio) -2
-1
0
1
2 at 392.356 kb on + strand at 392.404 kb on + strand at 392.405 kb on - strand at 392.497 kb on - strand at 392.520 kb on - strand at 392.718 kb on + strand at 392.718 kb on + strand at 392.718 kb on + strand at 392.718 kb on + strand at 392.892 kb on + strand at 392.892 kb on + strand at 392.922 kb on + strand at 393.015 kb on + strand, within BPHYT_RS01725 at 393.085 kb on + strand, within BPHYT_RS01725 at 393.085 kb on + strand, within BPHYT_RS01725 at 393.221 kb on + strand at 393.221 kb on + strand at 393.222 kb on - strand at 393.476 kb on + strand, within BPHYT_RS01730 at 393.521 kb on + strand, within BPHYT_RS01730 at 393.640 kb on + strand, within BPHYT_RS01730 at 393.641 kb on - strand, within BPHYT_RS01730 at 394.563 kb on + strand, within BPHYT_RS01735 at 394.564 kb on - strand, within BPHYT_RS01735 at 394.564 kb on - strand, within BPHYT_RS01735 at 394.564 kb on - strand, within BPHYT_RS01735 at 394.622 kb on + strand, within BPHYT_RS01735 at 394.623 kb on - strand, within BPHYT_RS01735 at 394.819 kb on + strand at 394.820 kb on - strand, within BPHYT_RS01735 at 394.820 kb on - strand, within BPHYT_RS01735 at 395.169 kb on + strand, within BPHYT_RS01735 at 395.170 kb on - strand, within BPHYT_RS01735 at 395.170 kb on - strand, within BPHYT_RS01735 at 395.194 kb on + strand, within BPHYT_RS01735 at 395.194 kb on + strand, within BPHYT_RS01735 at 395.194 kb on + strand, within BPHYT_RS01735 at 395.194 kb on + strand, within BPHYT_RS01735 at 395.195 kb on - strand, within BPHYT_RS01735 at 395.195 kb on - strand, within BPHYT_RS01735 at 395.195 kb on - strand, within BPHYT_RS01735 at 395.195 kb on - strand, within BPHYT_RS01735 at 395.308 kb on - strand, within BPHYT_RS01735
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 0.1 remove 392,356 + +0.5 392,404 + +0.3 392,405 - -0.2 392,497 - -0.4 392,520 - -0.1 392,718 + -0.3 392,718 + +0.0 392,718 + +1.3 392,718 + -1.6 392,892 + -0.9 392,892 + -0.8 392,922 + -0.5 393,015 + BPHYT_RS01725 0.15 -0.1 393,085 + BPHYT_RS01725 0.48 -0.6 393,085 + BPHYT_RS01725 0.48 -0.3 393,221 + -0.1 393,221 + +0.0 393,222 - -1.6 393,476 + BPHYT_RS01730 0.16 -1.3 393,521 + BPHYT_RS01730 0.20 +1.4 393,640 + BPHYT_RS01730 0.32 -1.2 393,641 - BPHYT_RS01730 0.32 +0.3 394,563 + BPHYT_RS01735 0.10 -1.0 394,564 - BPHYT_RS01735 0.10 -0.4 394,564 - BPHYT_RS01735 0.10 +1.3 394,564 - BPHYT_RS01735 0.10 +2.4 394,622 + BPHYT_RS01735 0.13 -1.2 394,623 - BPHYT_RS01735 0.13 -0.4 394,819 + +1.7 394,820 - BPHYT_RS01735 0.24 +0.3 394,820 - BPHYT_RS01735 0.24 +2.2 395,169 + BPHYT_RS01735 0.42 -1.1 395,170 - BPHYT_RS01735 0.42 +0.4 395,170 - BPHYT_RS01735 0.42 -1.0 395,194 + BPHYT_RS01735 0.43 -0.2 395,194 + BPHYT_RS01735 0.43 -0.5 395,194 + BPHYT_RS01735 0.43 -0.6 395,194 + BPHYT_RS01735 0.43 +0.2 395,195 - BPHYT_RS01735 0.44 +0.4 395,195 - BPHYT_RS01735 0.44 -0.3 395,195 - BPHYT_RS01735 0.44 -2.0 395,195 - BPHYT_RS01735 0.44 +0.5 395,308 - BPHYT_RS01735 0.49 -0.3
Or see this region's nucleotide sequence