Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36380 and BPHYT_RS36385 overlap by 4 nucleotides BPHYT_RS36385 and BPHYT_RS36390 overlap by 4 nucleotides BPHYT_RS36390 and BPHYT_RS36395 are separated by 84 nucleotides
BPHYT_RS36380: BPHYT_RS36380 - phage integrase, at 88,818 to 90,884
_RS36380
BPHYT_RS36385: BPHYT_RS36385 - TetR family transcriptional regulator, at 90,881 to 91,273
_RS36385
BPHYT_RS36390: BPHYT_RS36390 - hypothetical protein, at 91,270 to 91,524
_RS36390
BPHYT_RS36395: BPHYT_RS36395 - hypothetical protein, at 91,609 to 91,920
_RS36395
Position (kb) 90
91
92 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 90.002 kb on + strand, within BPHYT_RS36380 at 90.358 kb on - strand, within BPHYT_RS36380 at 90.358 kb on - strand at 90.848 kb on - strand at 91.173 kb on + strand, within BPHYT_RS36385 at 91.507 kb on + strand at 91.514 kb on - strand at 91.650 kb on + strand, within BPHYT_RS36395 at 91.651 kb on - strand, within BPHYT_RS36395 at 91.985 kb on - strand at 91.985 kb on - strand at 92.180 kb on + strand at 92.185 kb on - strand at 92.185 kb on - strand at 92.190 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 90,002 + BPHYT_RS36380 0.57 -0.7 90,358 - BPHYT_RS36380 0.75 -0.3 90,358 - +0.3 90,848 - -1.8 91,173 + BPHYT_RS36385 0.74 -1.1 91,507 + +1.9 91,514 - -1.5 91,650 + BPHYT_RS36395 0.13 +1.9 91,651 - BPHYT_RS36395 0.13 +0.3 91,985 - -1.1 91,985 - +0.5 92,180 + -2.8 92,185 - -0.1 92,185 - +1.1 92,190 + +0.8
Or see this region's nucleotide sequence