Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34970 and BPHYT_RS34975 overlap by 8 nucleotides BPHYT_RS34975 and BPHYT_RS34980 are separated by 40 nucleotides BPHYT_RS34980 and BPHYT_RS34985 are separated by 26 nucleotides BPHYT_RS34985 and BPHYT_RS34990 overlap by 8 nucleotides
BPHYT_RS34970: BPHYT_RS34970 - hypothetical protein, at 3,444,385 to 3,446,043
_RS34970
BPHYT_RS34975: BPHYT_RS34975 - L-aspartate dehydrogenase, at 3,446,036 to 3,446,866
_RS34975
BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397
_RS34980
BPHYT_RS34985: BPHYT_RS34985 - glyoxalase, at 3,448,424 to 3,449,389
_RS34985
BPHYT_RS34990: BPHYT_RS34990 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 3,449,382 to 3,450,647
_RS34990
Position (kb)
3446
3447
3448
3449 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.199 kb on + strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.865 kb on - strand at 3447.368 kb on + strand, within BPHYT_RS34980 at 3447.368 kb on + strand, within BPHYT_RS34980 at 3447.745 kb on - strand, within BPHYT_RS34980 at 3448.021 kb on - strand, within BPHYT_RS34980 at 3448.031 kb on + strand, within BPHYT_RS34980 at 3448.189 kb on - strand, within BPHYT_RS34980 at 3448.335 kb on + strand at 3448.395 kb on + strand at 3448.854 kb on - strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985 at 3449.113 kb on + strand, within BPHYT_RS34985
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,446,118 - -0.5 3,446,118 - -0.7 3,446,118 - -0.3 3,446,118 - -1.0 3,446,199 + BPHYT_RS34975 0.20 -0.2 3,446,493 - BPHYT_RS34975 0.55 -0.2 3,446,493 - BPHYT_RS34975 0.55 -0.6 3,446,865 - +0.9 3,447,368 + BPHYT_RS34980 0.31 -0.3 3,447,368 + BPHYT_RS34980 0.31 +0.7 3,447,745 - BPHYT_RS34980 0.56 +2.8 3,448,021 - BPHYT_RS34980 0.75 +0.0 3,448,031 + BPHYT_RS34980 0.75 -0.1 3,448,189 - BPHYT_RS34980 0.86 -1.2 3,448,335 + -1.5 3,448,395 + -0.4 3,448,854 - BPHYT_RS34985 0.45 -2.6 3,449,113 + BPHYT_RS34985 0.71 +0.1 3,449,113 + BPHYT_RS34985 0.71 -0.8
Or see this region's nucleotide sequence