Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34960 and BPHYT_RS34965 overlap by 1 nucleotides BPHYT_RS34965 and BPHYT_RS34970 overlap by 8 nucleotides BPHYT_RS34970 and BPHYT_RS34975 overlap by 8 nucleotides BPHYT_RS34975 and BPHYT_RS34980 are separated by 40 nucleotides
BPHYT_RS34960: BPHYT_RS34960 - IclR family transcriptional regulator, at 3,442,711 to 3,443,595
_RS34960
BPHYT_RS34965: BPHYT_RS34965 - short-chain dehydrogenase, at 3,443,595 to 3,444,392
_RS34965
BPHYT_RS34970: BPHYT_RS34970 - hypothetical protein, at 3,444,385 to 3,446,043
_RS34970
BPHYT_RS34975: BPHYT_RS34975 - L-aspartate dehydrogenase, at 3,446,036 to 3,446,866
_RS34975
BPHYT_RS34980: BPHYT_RS34980 - aldehyde dehydrogenase, at 3,446,907 to 3,448,397
_RS34980
Position (kb)
3444
3445
3446
3447 Strain fitness (log2 ratio)
-1
0
1
2 at 3443.536 kb on + strand at 3444.315 kb on + strand at 3444.324 kb on - strand at 3444.339 kb on + strand at 3444.727 kb on + strand, within BPHYT_RS34970 at 3445.175 kb on - strand, within BPHYT_RS34970 at 3445.175 kb on - strand, within BPHYT_RS34970 at 3445.238 kb on - strand, within BPHYT_RS34970 at 3445.775 kb on - strand, within BPHYT_RS34970 at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.118 kb on - strand at 3446.199 kb on + strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.493 kb on - strand, within BPHYT_RS34975 at 3446.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,443,536 + -0.9 3,444,315 + -0.9 3,444,324 - -0.5 3,444,339 + -1.3 3,444,727 + BPHYT_RS34970 0.21 +1.6 3,445,175 - BPHYT_RS34970 0.48 +0.5 3,445,175 - BPHYT_RS34970 0.48 -0.9 3,445,238 - BPHYT_RS34970 0.51 +0.4 3,445,775 - BPHYT_RS34970 0.84 +1.9 3,446,118 - -0.5 3,446,118 - -0.7 3,446,118 - -0.3 3,446,118 - -1.0 3,446,199 + BPHYT_RS34975 0.20 -0.2 3,446,493 - BPHYT_RS34975 0.55 -0.2 3,446,493 - BPHYT_RS34975 0.55 -0.6 3,446,865 - +0.9
Or see this region's nucleotide sequence