Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34945 and BPHYT_RS34950 are separated by 26 nucleotides BPHYT_RS34950 and BPHYT_RS34955 are separated by 45 nucleotides BPHYT_RS34955 and BPHYT_RS34960 are separated by 0 nucleotides BPHYT_RS34960 and BPHYT_RS34965 overlap by 1 nucleotides
BPHYT_RS34945: BPHYT_RS34945 - 3-ketoacyl-ACP reductase, at 3,440,453 to 3,441,220
_RS34945
BPHYT_RS34950: BPHYT_RS34950 - cupin, at 3,441,247 to 3,441,774
_RS34950
BPHYT_RS34955: BPHYT_RS34955 - alpha/beta hydrolase, at 3,441,820 to 3,442,710
_RS34955
BPHYT_RS34960: BPHYT_RS34960 - IclR family transcriptional regulator, at 3,442,711 to 3,443,595
_RS34960
BPHYT_RS34965: BPHYT_RS34965 - short-chain dehydrogenase, at 3,443,595 to 3,444,392
_RS34965
Position (kb) 3441
3442
3443 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3440.937 kb on - strand, within BPHYT_RS34945 at 3441.084 kb on - strand, within BPHYT_RS34945 at 3441.531 kb on + strand, within BPHYT_RS34950 at 3441.724 kb on - strand at 3441.724 kb on - strand at 3441.841 kb on - strand at 3443.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,440,937 - BPHYT_RS34945 0.63 -1.8 3,441,084 - BPHYT_RS34945 0.82 +2.4 3,441,531 + BPHYT_RS34950 0.54 -1.0 3,441,724 - +2.1 3,441,724 - -0.1 3,441,841 - +0.6 3,443,536 + -0.9
Or see this region's nucleotide sequence