Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34775 and BPHYT_RS34780 are separated by 277 nucleotides BPHYT_RS34780 and BPHYT_RS34785 are separated by 4 nucleotides
BPHYT_RS34775: BPHYT_RS34775 - diguanylate cyclase, at 3,405,749 to 3,406,687
_RS34775
BPHYT_RS34780: BPHYT_RS34780 - phytoene synthase, at 3,406,965 to 3,407,813
_RS34780
BPHYT_RS34785: BPHYT_RS34785 - hypothetical protein, at 3,407,818 to 3,409,086
_RS34785
Position (kb)
3406
3407
3408 Strain fitness (log2 ratio)
-2
-1
0
1 at 3406.194 kb on - strand, within BPHYT_RS34775 at 3406.262 kb on + strand, within BPHYT_RS34775 at 3406.641 kb on - strand at 3406.641 kb on - strand at 3406.643 kb on - strand at 3406.834 kb on + strand at 3406.836 kb on + strand at 3406.837 kb on - strand at 3406.837 kb on - strand at 3407.091 kb on + strand, within BPHYT_RS34780 at 3407.193 kb on + strand, within BPHYT_RS34780 at 3407.346 kb on + strand, within BPHYT_RS34780 at 3407.347 kb on - strand, within BPHYT_RS34780 at 3407.393 kb on - strand, within BPHYT_RS34780 at 3407.700 kb on + strand, within BPHYT_RS34780 at 3407.831 kb on + strand at 3407.831 kb on + strand at 3407.831 kb on + strand at 3407.831 kb on + strand at 3407.831 kb on + strand at 3407.950 kb on + strand, within BPHYT_RS34785 at 3408.025 kb on + strand, within BPHYT_RS34785 at 3408.123 kb on + strand, within BPHYT_RS34785 at 3408.123 kb on + strand, within BPHYT_RS34785 at 3408.123 kb on + strand, within BPHYT_RS34785 at 3408.123 kb on + strand, within BPHYT_RS34785 at 3408.196 kb on + strand, within BPHYT_RS34785
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,406,194 - BPHYT_RS34775 0.47 +0.1 3,406,262 + BPHYT_RS34775 0.55 -1.8 3,406,641 - -0.2 3,406,641 - -0.5 3,406,643 - -0.8 3,406,834 + +0.2 3,406,836 + -1.5 3,406,837 - -2.5 3,406,837 - -0.7 3,407,091 + BPHYT_RS34780 0.15 +1.3 3,407,193 + BPHYT_RS34780 0.27 -1.2 3,407,346 + BPHYT_RS34780 0.45 -2.7 3,407,347 - BPHYT_RS34780 0.45 +0.8 3,407,393 - BPHYT_RS34780 0.50 +1.3 3,407,700 + BPHYT_RS34780 0.87 -2.7 3,407,831 + +0.8 3,407,831 + -0.5 3,407,831 + +0.1 3,407,831 + +0.5 3,407,831 + -0.3 3,407,950 + BPHYT_RS34785 0.10 +0.2 3,408,025 + BPHYT_RS34785 0.16 +0.3 3,408,123 + BPHYT_RS34785 0.24 -0.3 3,408,123 + BPHYT_RS34785 0.24 -0.1 3,408,123 + BPHYT_RS34785 0.24 +0.4 3,408,123 + BPHYT_RS34785 0.24 +0.4 3,408,196 + BPHYT_RS34785 0.30 +0.4
Or see this region's nucleotide sequence