Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34300 and BPHYT_RS34305 are separated by 121 nucleotides BPHYT_RS34305 and BPHYT_RS34310 overlap by 4 nucleotides
BPHYT_RS34300: BPHYT_RS34300 - major facilitator transporter, at 3,300,173 to 3,301,555
_RS34300
BPHYT_RS34305: BPHYT_RS34305 - succinate-semialdehyde dehydrogenase, at 3,301,677 to 3,303,116
_RS34305
BPHYT_RS34310: BPHYT_RS34310 - NADP-dependent aryl-alcohol dehydrogenase, at 3,303,113 to 3,304,150
_RS34310
Position (kb)
3301
3302
3303
3304 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3300.682 kb on + strand, within BPHYT_RS34300 at 3300.683 kb on - strand, within BPHYT_RS34300 at 3300.907 kb on + strand, within BPHYT_RS34300 at 3300.993 kb on + strand, within BPHYT_RS34300 at 3301.182 kb on + strand, within BPHYT_RS34300 at 3301.736 kb on - strand at 3302.249 kb on - strand, within BPHYT_RS34305 at 3302.755 kb on + strand, within BPHYT_RS34305 at 3302.976 kb on - strand at 3303.052 kb on + strand at 3303.438 kb on + strand, within BPHYT_RS34310 at 3303.608 kb on - strand, within BPHYT_RS34310 at 3303.672 kb on + strand, within BPHYT_RS34310 at 3303.672 kb on + strand, within BPHYT_RS34310 at 3303.694 kb on - strand, within BPHYT_RS34310 at 3303.780 kb on + strand, within BPHYT_RS34310 at 3303.780 kb on + strand, within BPHYT_RS34310 at 3303.780 kb on + strand, within BPHYT_RS34310 at 3303.780 kb on + strand, within BPHYT_RS34310 at 3303.780 kb on + strand, within BPHYT_RS34310 at 3303.781 kb on - strand, within BPHYT_RS34310 at 3303.793 kb on - strand, within BPHYT_RS34310 at 3303.975 kb on + strand, within BPHYT_RS34310
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,300,682 + BPHYT_RS34300 0.37 -0.5 3,300,683 - BPHYT_RS34300 0.37 +0.8 3,300,907 + BPHYT_RS34300 0.53 +0.2 3,300,993 + BPHYT_RS34300 0.59 +0.3 3,301,182 + BPHYT_RS34300 0.73 +1.5 3,301,736 - +0.1 3,302,249 - BPHYT_RS34305 0.40 -0.8 3,302,755 + BPHYT_RS34305 0.75 -3.0 3,302,976 - +0.2 3,303,052 + -0.7 3,303,438 + BPHYT_RS34310 0.31 -0.2 3,303,608 - BPHYT_RS34310 0.48 -0.5 3,303,672 + BPHYT_RS34310 0.54 -1.2 3,303,672 + BPHYT_RS34310 0.54 -0.8 3,303,694 - BPHYT_RS34310 0.56 -0.4 3,303,780 + BPHYT_RS34310 0.64 +0.8 3,303,780 + BPHYT_RS34310 0.64 +0.6 3,303,780 + BPHYT_RS34310 0.64 +0.4 3,303,780 + BPHYT_RS34310 0.64 +0.0 3,303,780 + BPHYT_RS34310 0.64 -0.1 3,303,781 - BPHYT_RS34310 0.64 +0.6 3,303,793 - BPHYT_RS34310 0.66 +0.6 3,303,975 + BPHYT_RS34310 0.83 -1.3
Or see this region's nucleotide sequence