Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34100 and BPHYT_RS34105 are separated by 129 nucleotides BPHYT_RS34105 and BPHYT_RS34110 are separated by 6 nucleotides
BPHYT_RS34100: BPHYT_RS34100 - spermidine/putrescine ABC transporter ATP-binding protein, at 3,248,375 to 3,249,472
_RS34100
BPHYT_RS34105: BPHYT_RS34105 - decarboxylase, at 3,249,602 to 3,251,149
_RS34105
BPHYT_RS34110: BPHYT_RS34110 - hypothetical protein, at 3,251,156 to 3,254,899
_RS34110
Position (kb)
3249
3250
3251
3252 Strain fitness (log2 ratio)
-2
-1
0
1 at 3248.794 kb on - strand, within BPHYT_RS34100 at 3249.379 kb on - strand at 3249.379 kb on - strand at 3249.407 kb on + strand at 3249.423 kb on + strand at 3249.545 kb on + strand at 3249.545 kb on + strand at 3249.546 kb on - strand at 3249.547 kb on + strand at 3249.547 kb on + strand at 3249.563 kb on + strand at 3249.821 kb on + strand, within BPHYT_RS34105 at 3249.822 kb on - strand, within BPHYT_RS34105 at 3249.852 kb on - strand, within BPHYT_RS34105 at 3250.515 kb on - strand, within BPHYT_RS34105 at 3250.554 kb on - strand, within BPHYT_RS34105 at 3250.677 kb on - strand, within BPHYT_RS34105 at 3250.677 kb on - strand, within BPHYT_RS34105 at 3250.827 kb on - strand, within BPHYT_RS34105 at 3251.010 kb on - strand at 3251.233 kb on + strand at 3251.425 kb on - strand at 3251.425 kb on - strand at 3251.425 kb on - strand at 3251.425 kb on - strand at 3251.439 kb on + strand at 3251.439 kb on + strand at 3251.439 kb on + strand at 3251.439 kb on + strand at 3251.440 kb on - strand at 3251.440 kb on - strand at 3251.440 kb on - strand at 3251.448 kb on + strand at 3251.700 kb on + strand, within BPHYT_RS34110 at 3252.004 kb on - strand, within BPHYT_RS34110 at 3252.004 kb on - strand, within BPHYT_RS34110 at 3252.051 kb on + strand, within BPHYT_RS34110 at 3252.063 kb on + strand, within BPHYT_RS34110 at 3252.063 kb on + strand, within BPHYT_RS34110
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,248,794 - BPHYT_RS34100 0.38 +0.6 3,249,379 - -0.1 3,249,379 - -0.5 3,249,407 + +0.1 3,249,423 + -2.3 3,249,545 + -0.8 3,249,545 + +0.2 3,249,546 - -2.0 3,249,547 + -1.2 3,249,547 + -0.2 3,249,563 + -0.5 3,249,821 + BPHYT_RS34105 0.14 -0.2 3,249,822 - BPHYT_RS34105 0.14 -0.5 3,249,852 - BPHYT_RS34105 0.16 -0.6 3,250,515 - BPHYT_RS34105 0.59 +1.5 3,250,554 - BPHYT_RS34105 0.61 +0.4 3,250,677 - BPHYT_RS34105 0.69 -0.1 3,250,677 - BPHYT_RS34105 0.69 +0.7 3,250,827 - BPHYT_RS34105 0.79 -1.7 3,251,010 - -0.2 3,251,233 + +0.0 3,251,425 - -1.3 3,251,425 - -0.2 3,251,425 - -1.0 3,251,425 - -1.0 3,251,439 + -0.5 3,251,439 + -1.1 3,251,439 + -0.9 3,251,439 + -0.6 3,251,440 - -0.0 3,251,440 - -0.4 3,251,440 - -1.4 3,251,448 + -0.7 3,251,700 + BPHYT_RS34110 0.15 -1.7 3,252,004 - BPHYT_RS34110 0.23 -1.1 3,252,004 - BPHYT_RS34110 0.23 -1.3 3,252,051 + BPHYT_RS34110 0.24 -0.0 3,252,063 + BPHYT_RS34110 0.24 +0.0 3,252,063 + BPHYT_RS34110 0.24 +0.7
Or see this region's nucleotide sequence