Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34025

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34015 and BPHYT_RS34020 overlap by 1 nucleotidesBPHYT_RS34020 and BPHYT_RS34025 overlap by 4 nucleotidesBPHYT_RS34025 and BPHYT_RS34030 are separated by 234 nucleotides BPHYT_RS34015: BPHYT_RS34015 - hypothetical protein, at 3,230,709 to 3,232,235 _RS34015 BPHYT_RS34020: BPHYT_RS34020 - hypothetical protein, at 3,232,235 to 3,233,119 _RS34020 BPHYT_RS34025: BPHYT_RS34025 - hypothetical protein, at 3,233,116 to 3,233,835 _RS34025 BPHYT_RS34030: BPHYT_RS34030 - pilus assembly protein CpaB, at 3,234,070 to 3,235,170 _RS34030 Position (kb) 3233 3234Strain fitness (log2 ratio) -2 -1 0 1at 3232.249 kb on + strandat 3232.249 kb on + strandat 3232.484 kb on + strand, within BPHYT_RS34020at 3232.485 kb on - strand, within BPHYT_RS34020at 3232.507 kb on - strand, within BPHYT_RS34020at 3232.507 kb on - strand, within BPHYT_RS34020at 3232.721 kb on - strand, within BPHYT_RS34020at 3232.721 kb on - strand, within BPHYT_RS34020at 3232.934 kb on - strand, within BPHYT_RS34020at 3233.025 kb on - strand, within BPHYT_RS34020at 3233.091 kb on + strandat 3233.702 kb on - strand, within BPHYT_RS34025at 3233.982 kb on - strandat 3234.116 kb on + strandat 3234.137 kb on + strandat 3234.567 kb on - strand, within BPHYT_RS34030at 3234.569 kb on - strand, within BPHYT_RS34030at 3234.740 kb on - strand, within BPHYT_RS34030

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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3,232,249 + +0.5
3,232,249 + -0.4
3,232,484 + BPHYT_RS34020 0.28 -2.2
3,232,485 - BPHYT_RS34020 0.28 +0.9
3,232,507 - BPHYT_RS34020 0.31 -0.2
3,232,507 - BPHYT_RS34020 0.31 -0.6
3,232,721 - BPHYT_RS34020 0.55 -1.8
3,232,721 - BPHYT_RS34020 0.55 -0.7
3,232,934 - BPHYT_RS34020 0.79 +0.6
3,233,025 - BPHYT_RS34020 0.89 -0.7
3,233,091 + +0.0
3,233,702 - BPHYT_RS34025 0.81 +0.8
3,233,982 - -0.2
3,234,116 + +0.3
3,234,137 + -0.8
3,234,567 - BPHYT_RS34030 0.45 -1.5
3,234,569 - BPHYT_RS34030 0.45 -0.8
3,234,740 - BPHYT_RS34030 0.61 -0.4

Or see this region's nucleotide sequence