Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS31340 and BPHYT_RS31345 are separated by 14 nucleotides BPHYT_RS31345 and BPHYT_RS31350 are separated by 162 nucleotides BPHYT_RS31350 and BPHYT_RS31355 are separated by 286 nucleotides BPHYT_RS31355 and BPHYT_RS31360 are separated by 54 nucleotides
BPHYT_RS31340: BPHYT_RS31340 - malonate transporter MadL subunit, at 2,586,938 to 2,587,333
_RS31340
BPHYT_RS31345: BPHYT_RS31345 - malonate transporter, at 2,587,348 to 2,588,115
_RS31345
BPHYT_RS31350: BPHYT_RS31350 - LysR family transcriptional regulator, at 2,588,278 to 2,589,192
_RS31350
BPHYT_RS31355: BPHYT_RS31355 - hypothetical protein, at 2,589,479 to 2,589,787
_RS31355
BPHYT_RS31360: BPHYT_RS31360 - ATP-dependent DNA helicase RecQ, at 2,589,842 to 2,591,608
_RS31360
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2587.356 kb on + strand at 2587.790 kb on - strand, within BPHYT_RS31345 at 2587.790 kb on - strand, within BPHYT_RS31345 at 2588.116 kb on + strand at 2588.387 kb on + strand, within BPHYT_RS31350 at 2588.387 kb on + strand, within BPHYT_RS31350 at 2588.457 kb on + strand, within BPHYT_RS31350 at 2588.457 kb on + strand, within BPHYT_RS31350 at 2588.459 kb on + strand, within BPHYT_RS31350 at 2588.874 kb on - strand, within BPHYT_RS31350 at 2589.368 kb on - strand at 2590.147 kb on - strand, within BPHYT_RS31360
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 2,587,356 + +1.0 2,587,790 - BPHYT_RS31345 0.58 +0.2 2,587,790 - BPHYT_RS31345 0.58 -0.3 2,588,116 + +2.2 2,588,387 + BPHYT_RS31350 0.12 -0.8 2,588,387 + BPHYT_RS31350 0.12 -0.5 2,588,457 + BPHYT_RS31350 0.20 -2.0 2,588,457 + BPHYT_RS31350 0.20 +0.4 2,588,459 + BPHYT_RS31350 0.20 +0.5 2,588,874 - BPHYT_RS31350 0.65 -1.7 2,589,368 - +0.6 2,590,147 - BPHYT_RS31360 0.17 -0.6
Or see this region's nucleotide sequence