Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30835

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30830 and BPHYT_RS30835 are separated by 82 nucleotidesBPHYT_RS30835 and BPHYT_RS30840 are separated by 154 nucleotides BPHYT_RS30830: BPHYT_RS30830 - MFS transporter, at 2,472,546 to 2,473,859 _RS30830 BPHYT_RS30835: BPHYT_RS30835 - hypothetical protein, at 2,473,942 to 2,474,304 _RS30835 BPHYT_RS30840: BPHYT_RS30840 - small mechanosensitive ion channel protein MscS, at 2,474,459 to 2,475,673 _RS30840 Position (kb) 2473 2474 2475Strain fitness (log2 ratio) -3 -2 -1 0 1at 2473.888 kb on + strandat 2473.889 kb on - strandat 2473.892 kb on + strandat 2473.943 kb on + strandat 2474.427 kb on + strandat 2474.481 kb on + strandat 2474.527 kb on - strandat 2474.555 kb on + strandat 2475.190 kb on - strand, within BPHYT_RS30840at 2475.190 kb on - strand, within BPHYT_RS30840

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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2,473,888 + -0.3
2,473,889 - +0.3
2,473,892 + -2.0
2,473,943 + +1.1
2,474,427 + -1.6
2,474,481 + -0.7
2,474,527 - +1.4
2,474,555 + -0.1
2,475,190 - BPHYT_RS30840 0.60 +0.4
2,475,190 - BPHYT_RS30840 0.60 -2.9

Or see this region's nucleotide sequence