Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS30705 and BPHYT_RS30710 are separated by 38 nucleotides BPHYT_RS30710 and BPHYT_RS30715 are separated by 246 nucleotides BPHYT_RS30715 and BPHYT_RS30720 are separated by 79 nucleotides BPHYT_RS30720 and BPHYT_RS30725 are separated by 118 nucleotides BPHYT_RS30725 and BPHYT_RS30730 overlap by 2 nucleotides
BPHYT_RS30705: BPHYT_RS30705 - hypothetical protein, at 2,448,039 to 2,448,236
_RS30705
BPHYT_RS30710: BPHYT_RS30710 - hypothetical protein, at 2,448,275 to 2,448,532
_RS30710
BPHYT_RS30715: BPHYT_RS30715 - hypothetical protein, at 2,448,779 to 2,449,096
_RS30715
BPHYT_RS30720: BPHYT_RS30720 - hypothetical protein, at 2,449,176 to 2,449,529
_RS30720
BPHYT_RS30725: BPHYT_RS30725 - hypothetical protein, at 2,449,648 to 2,449,896
_RS30725
BPHYT_RS30730: BPHYT_RS30730 - MFS transporter, at 2,449,895 to 2,451,121
_RS30730
Position (kb)
2449
2450 Strain fitness (log2 ratio)
-2
-1
0
1 at 2448.249 kb on - strand at 2449.177 kb on + strand at 2449.245 kb on + strand, within BPHYT_RS30720 at 2449.327 kb on + strand, within BPHYT_RS30720 at 2449.562 kb on - strand at 2449.831 kb on - strand, within BPHYT_RS30725 at 2449.913 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 2,448,249 - -1.3 2,449,177 + +1.0 2,449,245 + BPHYT_RS30720 0.19 -1.8 2,449,327 + BPHYT_RS30720 0.43 -0.6 2,449,562 - +0.4 2,449,831 - BPHYT_RS30725 0.73 -1.2 2,449,913 - -0.8
Or see this region's nucleotide sequence