Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30005

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30000 and BPHYT_RS30005 are separated by 89 nucleotidesBPHYT_RS30005 and BPHYT_RS30010 are separated by 80 nucleotides BPHYT_RS30000: BPHYT_RS30000 - hypothetical protein, at 2,285,690 to 2,286,484 _RS30000 BPHYT_RS30005: BPHYT_RS30005 - hypothetical protein, at 2,286,574 to 2,286,834 _RS30005 BPHYT_RS30010: BPHYT_RS30010 - cell division protein, at 2,286,915 to 2,288,819 _RS30010 Position (kb) 2286 2287Strain fitness (log2 ratio) -1 0 1at 2285.751 kb on - strandat 2286.139 kb on - strand, within BPHYT_RS30000at 2286.139 kb on - strand, within BPHYT_RS30000at 2286.203 kb on + strand, within BPHYT_RS30000at 2286.204 kb on - strand, within BPHYT_RS30000at 2287.045 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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2,285,751 - +0.2
2,286,139 - BPHYT_RS30000 0.56 +0.3
2,286,139 - BPHYT_RS30000 0.56 -1.4
2,286,203 + BPHYT_RS30000 0.65 -0.2
2,286,204 - BPHYT_RS30000 0.65 -0.5
2,287,045 + -0.3

Or see this region's nucleotide sequence