Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28765

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28760 and BPHYT_RS28765 are separated by 81 nucleotidesBPHYT_RS28765 and BPHYT_RS28770 are separated by 33 nucleotides BPHYT_RS28760: BPHYT_RS28760 - porin, at 2,005,346 to 2,006,581 _RS28760 BPHYT_RS28765: BPHYT_RS28765 - fumarylacetoacetate hydrolase, at 2,006,663 to 2,007,847 _RS28765 BPHYT_RS28770: BPHYT_RS28770 - aldehyde dehydrogenase, at 2,007,881 to 2,009,323 _RS28770 Position (kb) 2006 2007 2008Strain fitness (log2 ratio) -2 -1 0 1at 2005.728 kb on - strand, within BPHYT_RS28760at 2006.174 kb on + strand, within BPHYT_RS28760at 2006.296 kb on + strand, within BPHYT_RS28760at 2006.300 kb on + strand, within BPHYT_RS28760at 2006.302 kb on + strand, within BPHYT_RS28760at 2006.373 kb on - strand, within BPHYT_RS28760at 2006.399 kb on + strand, within BPHYT_RS28760at 2006.540 kb on - strandat 2006.563 kb on + strandat 2007.174 kb on - strand, within BPHYT_RS28765at 2007.207 kb on + strand, within BPHYT_RS28765at 2007.550 kb on + strand, within BPHYT_RS28765at 2007.692 kb on - strand, within BPHYT_RS28765

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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2,005,728 - BPHYT_RS28760 0.31 +0.4
2,006,174 + BPHYT_RS28760 0.67 +1.3
2,006,296 + BPHYT_RS28760 0.77 -2.2
2,006,300 + BPHYT_RS28760 0.77 -0.6
2,006,302 + BPHYT_RS28760 0.77 -0.0
2,006,373 - BPHYT_RS28760 0.83 +0.1
2,006,399 + BPHYT_RS28760 0.85 +0.5
2,006,540 - -0.2
2,006,563 + -1.2
2,007,174 - BPHYT_RS28765 0.43 -0.3
2,007,207 + BPHYT_RS28765 0.46 -1.2
2,007,550 + BPHYT_RS28765 0.75 -2.3
2,007,692 - BPHYT_RS28765 0.87 -2.2

Or see this region's nucleotide sequence