Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28730 and BPHYT_RS28735 are separated by 160 nucleotides BPHYT_RS28735 and BPHYT_RS28740 are separated by 29 nucleotides BPHYT_RS28740 and BPHYT_RS28745 are separated by 13 nucleotides
BPHYT_RS28730: BPHYT_RS28730 - galactarate dehydratase, at 1,999,039 to 2,000,550
_RS28730
BPHYT_RS28735: BPHYT_RS28735 - hypothetical protein, at 2,000,711 to 2,001,406
_RS28735
BPHYT_RS28740: BPHYT_RS28740 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 2,001,436 to 2,002,326
_RS28740
BPHYT_RS28745: BPHYT_RS28745 - 2-keto-4-pentenoate hydratase, at 2,002,340 to 2,003,149
_RS28745
Position (kb) 2000
2001
2002 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1999.719 kb on + strand, within BPHYT_RS28730 at 2000.202 kb on - strand, within BPHYT_RS28730 at 2000.341 kb on + strand, within BPHYT_RS28730 at 2000.342 kb on - strand, within BPHYT_RS28730 at 2000.817 kb on - strand, within BPHYT_RS28735 at 2000.878 kb on + strand, within BPHYT_RS28735 at 2000.879 kb on - strand, within BPHYT_RS28735 at 2001.073 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.077 kb on - strand, within BPHYT_RS28735 at 2001.406 kb on + strand at 2001.438 kb on - strand at 2001.480 kb on + strand at 2001.489 kb on - strand at 2001.609 kb on + strand, within BPHYT_RS28740 at 2001.651 kb on - strand, within BPHYT_RS28740 at 2001.810 kb on + strand, within BPHYT_RS28740
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,999,719 + BPHYT_RS28730 0.45 +1.2 2,000,202 - BPHYT_RS28730 0.77 -0.3 2,000,341 + BPHYT_RS28730 0.86 -2.1 2,000,342 - BPHYT_RS28730 0.86 +0.5 2,000,817 - BPHYT_RS28735 0.15 +2.6 2,000,878 + BPHYT_RS28735 0.24 -0.4 2,000,879 - BPHYT_RS28735 0.24 -0.8 2,001,073 - BPHYT_RS28735 0.52 +0.7 2,001,077 - BPHYT_RS28735 0.53 +1.1 2,001,077 - BPHYT_RS28735 0.53 +0.1 2,001,406 + -2.6 2,001,438 - +0.6 2,001,480 + -1.3 2,001,489 - +0.2 2,001,609 + BPHYT_RS28740 0.19 -0.5 2,001,651 - BPHYT_RS28740 0.24 +1.9 2,001,810 + BPHYT_RS28740 0.42 +1.3
Or see this region's nucleotide sequence