Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS28040

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS28035 and BPHYT_RS28040 are separated by 111 nucleotidesBPHYT_RS28040 and BPHYT_RS28045 are separated by 270 nucleotides BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665 _RS28035 BPHYT_RS28040: BPHYT_RS28040 - acyl-CoA dehydrogenase, at 1,854,777 to 1,855,910 _RS28040 BPHYT_RS28045: BPHYT_RS28045 - AraC family transcriptional regulator, at 1,856,181 to 1,857,218 _RS28045 Position (kb) 1854 1855 1856Strain fitness (log2 ratio) -1 0 1at 1854.457 kb on + strand, within BPHYT_RS28035at 1854.604 kb on + strandat 1854.625 kb on - strandat 1854.690 kb on - strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1854.778 kb on + strandat 1855.310 kb on + strand, within BPHYT_RS28040at 1855.310 kb on + strand, within BPHYT_RS28040at 1855.310 kb on + strand, within BPHYT_RS28040at 1855.641 kb on - strand, within BPHYT_RS28040at 1855.723 kb on + strand, within BPHYT_RS28040at 1855.724 kb on - strand, within BPHYT_RS28040at 1855.897 kb on + strandat 1856.095 kb on + strandat 1856.095 kb on + strandat 1856.096 kb on - strandat 1856.096 kb on - strandat 1856.177 kb on - strandat 1856.181 kb on - strandat 1856.205 kb on + strandat 1856.337 kb on + strand, within BPHYT_RS28045at 1856.338 kb on - strand, within BPHYT_RS28045at 1856.506 kb on + strand, within BPHYT_RS28045at 1856.507 kb on - strand, within BPHYT_RS28045at 1856.761 kb on - strand, within BPHYT_RS28045at 1856.761 kb on - strand, within BPHYT_RS28045at 1856.862 kb on + strand, within BPHYT_RS28045

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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1,854,457 + BPHYT_RS28035 0.88 +0.7
1,854,604 + +0.9
1,854,625 - -0.4
1,854,690 - +1.4
1,854,778 + -0.3
1,854,778 + -0.1
1,854,778 + -0.7
1,854,778 + +0.7
1,855,310 + BPHYT_RS28040 0.47 -0.4
1,855,310 + BPHYT_RS28040 0.47 +1.3
1,855,310 + BPHYT_RS28040 0.47 +0.3
1,855,641 - BPHYT_RS28040 0.76 +0.4
1,855,723 + BPHYT_RS28040 0.83 +0.7
1,855,724 - BPHYT_RS28040 0.84 -1.5
1,855,897 + -0.9
1,856,095 + +0.1
1,856,095 + +0.4
1,856,096 - -0.1
1,856,096 - +0.8
1,856,177 - -0.7
1,856,181 - +0.4
1,856,205 + -1.0
1,856,337 + BPHYT_RS28045 0.15 -1.1
1,856,338 - BPHYT_RS28045 0.15 -0.5
1,856,506 + BPHYT_RS28045 0.31 -0.1
1,856,507 - BPHYT_RS28045 0.31 +0.4
1,856,761 - BPHYT_RS28045 0.56 -1.6
1,856,761 - BPHYT_RS28045 0.56 +1.3
1,856,862 + BPHYT_RS28045 0.66 -1.2

Or see this region's nucleotide sequence