Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28020 and BPHYT_RS28025 overlap by 4 nucleotides BPHYT_RS28025 and BPHYT_RS28030 are separated by 24 nucleotides BPHYT_RS28030 and BPHYT_RS28035 are separated by 72 nucleotides
BPHYT_RS28020: BPHYT_RS28020 - enoyl-CoA hydratase, at 1,849,636 to 1,850,454
_RS28020
BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365
_RS28025
BPHYT_RS28030: BPHYT_RS28030 - methylmalonate-semialdehyde dehydrogenase, at 1,851,390 to 1,852,901
_RS28030
BPHYT_RS28035: BPHYT_RS28035 - AMP-dependent synthetase, at 1,852,974 to 1,854,665
_RS28035
Position (kb) 1851
1852
1853 Strain fitness (log2 ratio) -1
0
1
2 at 1850.438 kb on + strand at 1850.713 kb on + strand, within BPHYT_RS28025 at 1850.938 kb on + strand, within BPHYT_RS28025 at 1851.282 kb on + strand at 1851.500 kb on - strand at 1852.483 kb on + strand, within BPHYT_RS28030 at 1852.483 kb on + strand, within BPHYT_RS28030 at 1852.484 kb on - strand, within BPHYT_RS28030 at 1852.484 kb on - strand, within BPHYT_RS28030 at 1853.098 kb on - strand at 1853.660 kb on + strand, within BPHYT_RS28035
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,850,438 + -0.3 1,850,713 + BPHYT_RS28025 0.29 -0.8 1,850,938 + BPHYT_RS28025 0.53 -0.2 1,851,282 + -0.6 1,851,500 - +2.1 1,852,483 + BPHYT_RS28030 0.72 -0.1 1,852,483 + BPHYT_RS28030 0.72 +1.3 1,852,484 - BPHYT_RS28030 0.72 +1.4 1,852,484 - BPHYT_RS28030 0.72 +0.1 1,853,098 - +1.7 1,853,660 + BPHYT_RS28035 0.41 +0.9
Or see this region's nucleotide sequence