Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS28005 and BPHYT_RS28010 are separated by 78 nucleotides BPHYT_RS28010 and BPHYT_RS28015 are separated by 545 nucleotides BPHYT_RS28015 and BPHYT_RS28020 overlap by 4 nucleotides BPHYT_RS28020 and BPHYT_RS28025 overlap by 4 nucleotides
BPHYT_RS28005: BPHYT_RS28005 - hypothetical protein, at 1,847,442 to 1,847,684
_RS28005
BPHYT_RS28010: BPHYT_RS28010 - hypothetical protein, at 1,847,763 to 1,847,945
_RS28010
BPHYT_RS28015: BPHYT_RS28015 - enoyl-CoA hydratase, at 1,848,491 to 1,849,639
_RS28015
BPHYT_RS28020: BPHYT_RS28020 - enoyl-CoA hydratase, at 1,849,636 to 1,850,454
_RS28020
BPHYT_RS28025: BPHYT_RS28025 - 3-hydroxyisobutyrate dehydrogenase, at 1,850,451 to 1,851,365
_RS28025
Position (kb) 1848
1849
1850 Strain fitness (log2 ratio) -2
-1
0
1 at 1848.227 kb on + strand at 1848.227 kb on + strand at 1848.228 kb on - strand at 1848.331 kb on + strand at 1848.756 kb on + strand, within BPHYT_RS28015 at 1848.793 kb on - strand, within BPHYT_RS28015 at 1848.793 kb on - strand, within BPHYT_RS28015 at 1849.117 kb on - strand, within BPHYT_RS28015 at 1850.438 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,848,227 + +0.0 1,848,227 + -0.9 1,848,228 - +0.1 1,848,331 + -1.7 1,848,756 + BPHYT_RS28015 0.23 -0.8 1,848,793 - BPHYT_RS28015 0.26 -0.9 1,848,793 - BPHYT_RS28015 0.26 -2.3 1,849,117 - BPHYT_RS28015 0.54 -1.9 1,850,438 + -0.3
Or see this region's nucleotide sequence