Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS27575 and BPHYT_RS27580 overlap by 4 nucleotides BPHYT_RS27580 and BPHYT_RS27585 overlap by 14 nucleotides BPHYT_RS27585 and BPHYT_RS27590 are separated by 9 nucleotides BPHYT_RS27590 and BPHYT_RS27595 overlap by 4 nucleotides
BPHYT_RS27575: BPHYT_RS27575 - ATP synthase F1 subunit gamma, at 1,759,233 to 1,760,081
_RS27575
BPHYT_RS27580: BPHYT_RS27580 - F0F1 ATP synthase subunit alpha, at 1,760,078 to 1,761,646
_RS27580
BPHYT_RS27585: BPHYT_RS27585 - ATP synthase F0 subunit B, at 1,761,633 to 1,762,382
_RS27585
BPHYT_RS27590: BPHYT_RS27590 - F0F1 ATP synthase subunit C, at 1,762,392 to 1,762,640
_RS27590
BPHYT_RS27595: BPHYT_RS27595 - F0F1 ATP synthase subunit A, at 1,762,637 to 1,763,335
_RS27595
Position (kb) 1760
1761
1762 Strain fitness (log2 ratio) -1
0
1 at 1759.429 kb on + strand, within BPHYT_RS27575 at 1759.429 kb on + strand, within BPHYT_RS27575 at 1759.435 kb on + strand, within BPHYT_RS27575 at 1759.560 kb on - strand, within BPHYT_RS27575 at 1759.788 kb on + strand, within BPHYT_RS27575 at 1760.481 kb on + strand, within BPHYT_RS27580 at 1760.895 kb on + strand, within BPHYT_RS27580 at 1760.995 kb on - strand, within BPHYT_RS27580 at 1762.444 kb on + strand, within BPHYT_RS27590
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,759,429 + BPHYT_RS27575 0.23 -1.5 1,759,429 + BPHYT_RS27575 0.23 +0.1 1,759,435 + BPHYT_RS27575 0.24 -0.0 1,759,560 - BPHYT_RS27575 0.39 +0.3 1,759,788 + BPHYT_RS27575 0.65 +0.7 1,760,481 + BPHYT_RS27580 0.26 +0.7 1,760,895 + BPHYT_RS27580 0.52 +1.6 1,760,995 - BPHYT_RS27580 0.58 -0.0 1,762,444 + BPHYT_RS27590 0.21 +1.3
Or see this region's nucleotide sequence