Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25875 and BPHYT_RS25880 are separated by 1 nucleotides BPHYT_RS25880 and BPHYT_RS25885 are separated by 32 nucleotides BPHYT_RS25885 and BPHYT_RS25890 overlap by 1 nucleotides BPHYT_RS25890 and BPHYT_RS25895 overlap by 4 nucleotides BPHYT_RS25895 and BPHYT_RS25900 are separated by 38 nucleotides
BPHYT_RS25875: BPHYT_RS25875 - type III secretion protein, at 1,384,937 to 1,387,012
_RS25875
BPHYT_RS25880: BPHYT_RS25880 - type III secretion protein, at 1,387,014 to 1,387,754
_RS25880
BPHYT_RS25885: BPHYT_RS25885 - type III secretion protein, at 1,387,787 to 1,388,476
_RS25885
BPHYT_RS25890: BPHYT_RS25890 - type III secretion protein, at 1,388,476 to 1,388,751
_RS25890
BPHYT_RS25895: BPHYT_RS25895 - type III secretion system protein, at 1,388,748 to 1,389,395
_RS25895
BPHYT_RS25900: BPHYT_RS25900 - hypothetical protein, at 1,389,434 to 1,390,375
_RS25900
Position (kb) 1387
1388
1389 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1386.885 kb on - strand at 1387.760 kb on + strand at 1387.760 kb on + strand at 1388.173 kb on - strand, within BPHYT_RS25885 at 1388.463 kb on - strand at 1389.112 kb on - strand, within BPHYT_RS25895
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,386,885 - -3.0 1,387,760 + +0.6 1,387,760 + -1.5 1,388,173 - BPHYT_RS25885 0.56 -0.5 1,388,463 - +0.7 1,389,112 - BPHYT_RS25895 0.56 -1.1
Or see this region's nucleotide sequence