Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25720 and BPHYT_RS25725 are separated by 98 nucleotides BPHYT_RS25725 and BPHYT_RS25730 overlap by 8 nucleotides BPHYT_RS25730 and BPHYT_RS25735 overlap by 4 nucleotides
BPHYT_RS25720: BPHYT_RS25720 - hypothetical protein, at 1,343,617 to 1,344,117
_RS25720
BPHYT_RS25725: BPHYT_RS25725 - hypothetical protein, at 1,344,216 to 1,344,713
_RS25725
BPHYT_RS25730: BPHYT_RS25730 - hypothetical protein, at 1,344,706 to 1,347,030
_RS25730
BPHYT_RS25735: BPHYT_RS25735 - integrase, at 1,347,027 to 1,348,070
_RS25735
Position (kb) 1344
1345
1346
1347
1348 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.879 kb on + strand, within BPHYT_RS25720 at 1343.920 kb on - strand, within BPHYT_RS25720 at 1344.123 kb on - strand at 1344.123 kb on - strand at 1344.408 kb on + strand, within BPHYT_RS25725 at 1344.535 kb on + strand at 1344.800 kb on - strand at 1345.253 kb on + strand, within BPHYT_RS25730 at 1345.312 kb on + strand, within BPHYT_RS25730 at 1345.663 kb on - strand, within BPHYT_RS25730 at 1345.663 kb on - strand, within BPHYT_RS25730 at 1345.921 kb on + strand, within BPHYT_RS25730 at 1345.921 kb on + strand, within BPHYT_RS25730 at 1345.921 kb on + strand, within BPHYT_RS25730 at 1345.929 kb on + strand, within BPHYT_RS25730 at 1346.698 kb on + strand, within BPHYT_RS25730 at 1346.699 kb on - strand, within BPHYT_RS25730 at 1346.699 kb on - strand, within BPHYT_RS25730 at 1347.066 kb on + strand at 1347.241 kb on + strand, within BPHYT_RS25735 at 1347.392 kb on - strand, within BPHYT_RS25735 at 1347.393 kb on + strand, within BPHYT_RS25735 at 1347.825 kb on + strand, within BPHYT_RS25735
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,343,879 + BPHYT_RS25720 0.52 -1.3 1,343,879 + BPHYT_RS25720 0.52 -0.3 1,343,879 + BPHYT_RS25720 0.52 -3.2 1,343,920 - BPHYT_RS25720 0.60 +1.0 1,344,123 - -0.7 1,344,123 - -0.3 1,344,408 + BPHYT_RS25725 0.39 +0.9 1,344,535 + +1.9 1,344,800 - -1.9 1,345,253 + BPHYT_RS25730 0.24 -0.0 1,345,312 + BPHYT_RS25730 0.26 +0.3 1,345,663 - BPHYT_RS25730 0.41 +1.0 1,345,663 - BPHYT_RS25730 0.41 +0.9 1,345,921 + BPHYT_RS25730 0.52 -0.4 1,345,921 + BPHYT_RS25730 0.52 +0.8 1,345,921 + BPHYT_RS25730 0.52 -1.8 1,345,929 + BPHYT_RS25730 0.53 +3.6 1,346,698 + BPHYT_RS25730 0.86 -0.3 1,346,699 - BPHYT_RS25730 0.86 +0.4 1,346,699 - BPHYT_RS25730 0.86 +0.9 1,347,066 + -0.9 1,347,241 + BPHYT_RS25735 0.20 -0.9 1,347,392 - BPHYT_RS25735 0.35 -0.5 1,347,393 + BPHYT_RS25735 0.35 -0.2 1,347,825 + BPHYT_RS25735 0.76 +1.2
Or see this region's nucleotide sequence