Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24660 and BPHYT_RS24665 are separated by 35 nucleotides BPHYT_RS24665 and BPHYT_RS24670 overlap by 4 nucleotides BPHYT_RS24670 and BPHYT_RS24675 overlap by 4 nucleotides
BPHYT_RS24660: BPHYT_RS24660 - ABC transporter ATP-binding protein, at 1,102,382 to 1,103,455
_RS24660
BPHYT_RS24665: BPHYT_RS24665 - ABC transporter permease, at 1,103,491 to 1,104,378
_RS24665
BPHYT_RS24670: BPHYT_RS24670 - ABC transporter permease, at 1,104,375 to 1,105,334
_RS24670
BPHYT_RS24675: BPHYT_RS24675 - spermidine/putrescine ABC transporter substrate-binding protein, at 1,105,331 to 1,106,425
_RS24675
Position (kb) 1104
1105
1106 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1103.444 kb on + strand at 1104.024 kb on - strand, within BPHYT_RS24665 at 1104.615 kb on + strand, within BPHYT_RS24670 at 1104.796 kb on + strand, within BPHYT_RS24670 at 1105.353 kb on + strand at 1105.635 kb on + strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.636 kb on - strand, within BPHYT_RS24675 at 1105.696 kb on - strand, within BPHYT_RS24675 at 1105.932 kb on + strand, within BPHYT_RS24675 at 1105.968 kb on + strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1105.969 kb on - strand, within BPHYT_RS24675 at 1106.249 kb on - strand, within BPHYT_RS24675
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,103,444 + -2.0 1,104,024 - BPHYT_RS24665 0.60 -0.4 1,104,615 + BPHYT_RS24670 0.25 -1.9 1,104,796 + BPHYT_RS24670 0.44 -0.8 1,105,353 + -1.2 1,105,635 + BPHYT_RS24675 0.28 -0.8 1,105,636 - BPHYT_RS24675 0.28 +2.2 1,105,636 - BPHYT_RS24675 0.28 -0.2 1,105,696 - BPHYT_RS24675 0.33 +0.6 1,105,932 + BPHYT_RS24675 0.55 +0.6 1,105,968 + BPHYT_RS24675 0.58 -0.7 1,105,969 - BPHYT_RS24675 0.58 -1.9 1,105,969 - BPHYT_RS24675 0.58 -1.1 1,106,249 - BPHYT_RS24675 0.84 -0.7
Or see this region's nucleotide sequence