Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22950 and BPHYT_RS22955 are separated by 7 nucleotides BPHYT_RS22955 and BPHYT_RS22960 are separated by 63 nucleotides BPHYT_RS22960 and BPHYT_RS22965 are separated by 74 nucleotides
BPHYT_RS22950: BPHYT_RS22950 - von Willebrand factor A, at 706,467 to 707,822
_RS22950
BPHYT_RS22955: BPHYT_RS22955 - isoprenylcysteine carboxyl methyltransferase, at 707,830 to 708,543
_RS22955
BPHYT_RS22960: BPHYT_RS22960 - peptidase, at 708,607 to 710,145
_RS22960
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
Position (kb) 708
709
710
711 Strain fitness (log2 ratio) -2
-1
0
1 at 707.743 kb on - strand at 707.811 kb on - strand at 708.161 kb on - strand, within BPHYT_RS22955 at 708.325 kb on + strand, within BPHYT_RS22955 at 708.359 kb on - strand, within BPHYT_RS22955 at 708.548 kb on - strand at 709.994 kb on + strand at 709.995 kb on - strand at 710.273 kb on - strand at 710.341 kb on + strand at 710.483 kb on - strand, within BPHYT_RS22965 at 710.527 kb on + strand, within BPHYT_RS22965 at 710.767 kb on - strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.808 kb on + strand, within BPHYT_RS22965 at 710.997 kb on - strand, within BPHYT_RS22965 at 710.998 kb on + strand, within BPHYT_RS22965
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 707,743 - -0.3 707,811 - +0.1 708,161 - BPHYT_RS22955 0.46 -1.9 708,325 + BPHYT_RS22955 0.69 -1.4 708,359 - BPHYT_RS22955 0.74 -0.4 708,548 - -0.4 709,994 + -1.5 709,995 - +0.1 710,273 - -1.7 710,341 + -0.4 710,483 - BPHYT_RS22965 0.11 +0.8 710,527 + BPHYT_RS22965 0.13 -2.0 710,767 - BPHYT_RS22965 0.23 +0.1 710,808 + BPHYT_RS22965 0.24 -1.4 710,808 + BPHYT_RS22965 0.24 -0.4 710,997 - BPHYT_RS22965 0.32 -0.6 710,998 + BPHYT_RS22965 0.32 -2.0
Or see this region's nucleotide sequence