Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22815

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22805 and BPHYT_RS22810 overlap by 4 nucleotidesBPHYT_RS22810 and BPHYT_RS22815 are separated by 26 nucleotidesBPHYT_RS22815 and BPHYT_RS22820 are separated by 561 nucleotides BPHYT_RS22805: BPHYT_RS22805 - biopolymer transporter ExbD, at 676,201 to 676,599 _RS22805 BPHYT_RS22810: BPHYT_RS22810 - biopolymer transporter ExbB, at 676,596 to 677,243 _RS22810 BPHYT_RS22815: BPHYT_RS22815 - TonB-denpendent receptor, at 677,270 to 679,615 _RS22815 BPHYT_RS22820: BPHYT_RS22820 - hydrolase, at 680,177 to 680,971 _RS22820 Position (kb) 677 678 679 680Strain fitness (log2 ratio) -2 -1 0 1at 677.006 kb on + strand, within BPHYT_RS22810at 677.006 kb on + strand, within BPHYT_RS22810at 677.438 kb on + strandat 677.616 kb on + strand, within BPHYT_RS22815at 677.763 kb on + strand, within BPHYT_RS22815at 678.099 kb on + strand, within BPHYT_RS22815at 680.499 kb on + strand, within BPHYT_RS22820at 680.550 kb on + strand, within BPHYT_RS22820

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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677,006 + BPHYT_RS22810 0.63 -2.6
677,006 + BPHYT_RS22810 0.63 +1.2
677,438 + +0.3
677,616 + BPHYT_RS22815 0.15 -1.2
677,763 + BPHYT_RS22815 0.21 -1.8
678,099 + BPHYT_RS22815 0.35 -1.9
680,499 + BPHYT_RS22820 0.41 +0.4
680,550 + BPHYT_RS22820 0.47 -0.9

Or see this region's nucleotide sequence