Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20425 and BPHYT_RS20430 are separated by 252 nucleotides BPHYT_RS20430 and BPHYT_RS20435 are separated by 60 nucleotides BPHYT_RS20435 and BPHYT_RS20440 are separated by 238 nucleotides
BPHYT_RS20425: BPHYT_RS20425 - hypothetical protein, at 146,078 to 146,386
_RS20425
BPHYT_RS20430: BPHYT_RS20430 - 3-dehydroquinate dehydratase, at 146,639 to 147,094
_RS20430
BPHYT_RS20435: BPHYT_RS20435 - MFS transporter, at 147,155 to 148,531
_RS20435
BPHYT_RS20440: BPHYT_RS20440 - IclR family transcriptional regulator, at 148,770 to 149,552
_RS20440
Position (kb) 147
148
149 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 146.304 kb on - strand, within BPHYT_RS20425 at 146.307 kb on - strand, within BPHYT_RS20425 at 146.466 kb on + strand at 146.466 kb on + strand at 146.467 kb on - strand at 146.467 kb on - strand at 146.509 kb on - strand at 146.596 kb on + strand at 146.596 kb on + strand at 146.597 kb on - strand at 146.792 kb on - strand, within BPHYT_RS20430 at 146.792 kb on - strand, within BPHYT_RS20430 at 147.074 kb on + strand at 147.075 kb on - strand at 147.075 kb on - strand at 147.078 kb on + strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.079 kb on - strand at 147.080 kb on + strand at 147.080 kb on + strand at 147.153 kb on + strand at 147.156 kb on + strand at 147.190 kb on + strand at 147.402 kb on + strand, within BPHYT_RS20435 at 147.556 kb on - strand, within BPHYT_RS20435 at 147.619 kb on + strand, within BPHYT_RS20435 at 147.753 kb on + strand, within BPHYT_RS20435 at 147.908 kb on - strand, within BPHYT_RS20435 at 147.909 kb on + strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.910 kb on - strand, within BPHYT_RS20435 at 147.964 kb on - strand, within BPHYT_RS20435 at 148.065 kb on + strand, within BPHYT_RS20435 at 148.065 kb on + strand, within BPHYT_RS20435 at 148.066 kb on - strand, within BPHYT_RS20435 at 148.185 kb on - strand, within BPHYT_RS20435 at 148.675 kb on - strand at 149.426 kb on + strand, within BPHYT_RS20440
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 146,304 - BPHYT_RS20425 0.73 +0.2 146,307 - BPHYT_RS20425 0.74 +0.8 146,466 + -0.7 146,466 + -0.4 146,467 - +2.2 146,467 - +0.1 146,509 - +1.2 146,596 + -0.5 146,596 + -0.1 146,597 - -0.8 146,792 - BPHYT_RS20430 0.34 +0.1 146,792 - BPHYT_RS20430 0.34 +0.2 147,074 + +1.1 147,075 - -0.2 147,075 - -1.4 147,078 + -1.2 147,079 - +0.4 147,079 - -1.2 147,079 - -0.4 147,079 - -0.2 147,080 + +0.7 147,080 + -0.1 147,153 + +0.6 147,156 + +0.1 147,190 + +0.2 147,402 + BPHYT_RS20435 0.18 +1.4 147,556 - BPHYT_RS20435 0.29 +1.1 147,619 + BPHYT_RS20435 0.34 -0.9 147,753 + BPHYT_RS20435 0.43 -1.6 147,908 - BPHYT_RS20435 0.55 +0.3 147,909 + BPHYT_RS20435 0.55 -1.6 147,910 - BPHYT_RS20435 0.55 +0.1 147,910 - BPHYT_RS20435 0.55 -0.0 147,910 - BPHYT_RS20435 0.55 +1.6 147,964 - BPHYT_RS20435 0.59 -0.7 148,065 + BPHYT_RS20435 0.66 -0.3 148,065 + BPHYT_RS20435 0.66 +0.7 148,066 - BPHYT_RS20435 0.66 +1.0 148,185 - BPHYT_RS20435 0.75 -2.1 148,675 - -0.0 149,426 + BPHYT_RS20440 0.84 -2.8
Or see this region's nucleotide sequence