Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19695 and BPHYT_RS19700 are separated by 23 nucleotides BPHYT_RS19700 and BPHYT_RS19705 are separated by 141 nucleotides BPHYT_RS19705 and BPHYT_RS19710 are separated by 188 nucleotides
BPHYT_RS19695: BPHYT_RS19695 - lactoylglutathione lyase, at 4,432,103 to 4,432,528
_RS19695
BPHYT_RS19700: BPHYT_RS19700 - hypothetical protein, at 4,432,552 to 4,433,106
_RS19700
BPHYT_RS19705: BPHYT_RS19705 - LysR family transcriptional regulator, at 4,433,248 to 4,434,225
_RS19705
BPHYT_RS19710: BPHYT_RS19710 - aldehyde dehydrogenase, at 4,434,414 to 4,435,862
_RS19710
Position (kb)
4433
4434
4435 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4432.324 kb on + strand at 4432.325 kb on - strand, within BPHYT_RS19695 at 4432.454 kb on + strand, within BPHYT_RS19695 at 4432.454 kb on + strand, within BPHYT_RS19695 at 4432.455 kb on - strand, within BPHYT_RS19695 at 4432.534 kb on - strand at 4432.534 kb on - strand at 4432.534 kb on - strand at 4432.534 kb on - strand at 4432.714 kb on + strand, within BPHYT_RS19700 at 4432.731 kb on + strand, within BPHYT_RS19700 at 4432.731 kb on + strand, within BPHYT_RS19700 at 4432.732 kb on - strand, within BPHYT_RS19700 at 4432.788 kb on - strand, within BPHYT_RS19700 at 4432.788 kb on - strand, within BPHYT_RS19700 at 4433.042 kb on + strand, within BPHYT_RS19700 at 4433.043 kb on - strand, within BPHYT_RS19700 at 4433.043 kb on - strand, within BPHYT_RS19700 at 4433.045 kb on + strand, within BPHYT_RS19700 at 4433.046 kb on - strand, within BPHYT_RS19700 at 4433.046 kb on - strand, within BPHYT_RS19700 at 4433.046 kb on - strand, within BPHYT_RS19700 at 4433.046 kb on - strand at 4433.084 kb on + strand at 4433.192 kb on + strand at 4433.193 kb on - strand at 4433.231 kb on + strand at 4433.250 kb on - strand at 4433.250 kb on - strand at 4433.283 kb on - strand at 4433.364 kb on + strand, within BPHYT_RS19705 at 4433.512 kb on - strand, within BPHYT_RS19705 at 4433.575 kb on + strand, within BPHYT_RS19705 at 4433.575 kb on + strand, within BPHYT_RS19705 at 4433.576 kb on - strand, within BPHYT_RS19705 at 4433.576 kb on - strand, within BPHYT_RS19705 at 4433.596 kb on + strand, within BPHYT_RS19705 at 4433.743 kb on + strand, within BPHYT_RS19705 at 4433.794 kb on + strand, within BPHYT_RS19705 at 4433.794 kb on + strand, within BPHYT_RS19705 at 4433.794 kb on + strand, within BPHYT_RS19705 at 4433.795 kb on - strand, within BPHYT_RS19705 at 4433.795 kb on - strand, within BPHYT_RS19705 at 4433.795 kb on - strand, within BPHYT_RS19705 at 4433.795 kb on - strand, within BPHYT_RS19705 at 4433.795 kb on - strand, within BPHYT_RS19705 at 4433.795 kb on - strand at 4434.036 kb on + strand, within BPHYT_RS19705 at 4434.167 kb on - strand at 4434.213 kb on - strand at 4434.213 kb on - strand at 4434.217 kb on - strand at 4434.217 kb on - strand at 4434.217 kb on - strand at 4434.366 kb on + strand at 4434.366 kb on + strand at 4434.367 kb on - strand at 4434.394 kb on + strand at 4434.394 kb on + strand at 4434.394 kb on + strand at 4434.395 kb on - strand at 4434.395 kb on - strand at 4434.409 kb on - strand at 4434.462 kb on + strand at 4434.462 kb on + strand at 4434.463 kb on - strand at 4434.463 kb on - strand at 4434.543 kb on + strand at 4434.543 kb on + strand at 4434.544 kb on - strand at 4434.573 kb on - strand, within BPHYT_RS19710
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 4,432,324 + -0.5 4,432,325 - BPHYT_RS19695 0.52 -1.3 4,432,454 + BPHYT_RS19695 0.82 -0.3 4,432,454 + BPHYT_RS19695 0.82 -0.4 4,432,455 - BPHYT_RS19695 0.83 +0.3 4,432,534 - -0.2 4,432,534 - +0.7 4,432,534 - -0.6 4,432,534 - +1.3 4,432,714 + BPHYT_RS19700 0.29 +0.1 4,432,731 + BPHYT_RS19700 0.32 +2.3 4,432,731 + BPHYT_RS19700 0.32 +0.0 4,432,732 - BPHYT_RS19700 0.32 +0.2 4,432,788 - BPHYT_RS19700 0.43 -0.0 4,432,788 - BPHYT_RS19700 0.43 +0.8 4,433,042 + BPHYT_RS19700 0.88 -0.7 4,433,043 - BPHYT_RS19700 0.88 -0.7 4,433,043 - BPHYT_RS19700 0.88 -0.6 4,433,045 + BPHYT_RS19700 0.89 +0.2 4,433,046 - BPHYT_RS19700 0.89 +1.0 4,433,046 - BPHYT_RS19700 0.89 +0.3 4,433,046 - BPHYT_RS19700 0.89 -0.2 4,433,046 - -0.7 4,433,084 + -1.7 4,433,192 + +1.4 4,433,193 - -1.0 4,433,231 + -0.6 4,433,250 - +0.0 4,433,250 - +0.1 4,433,283 - +0.7 4,433,364 + BPHYT_RS19705 0.12 -0.2 4,433,512 - BPHYT_RS19705 0.27 -0.9 4,433,575 + BPHYT_RS19705 0.33 -0.2 4,433,575 + BPHYT_RS19705 0.33 -0.9 4,433,576 - BPHYT_RS19705 0.34 -2.8 4,433,576 - BPHYT_RS19705 0.34 -0.8 4,433,596 + BPHYT_RS19705 0.36 +1.5 4,433,743 + BPHYT_RS19705 0.51 +1.3 4,433,794 + BPHYT_RS19705 0.56 -1.2 4,433,794 + BPHYT_RS19705 0.56 -0.3 4,433,794 + BPHYT_RS19705 0.56 -0.7 4,433,795 - BPHYT_RS19705 0.56 -2.0 4,433,795 - BPHYT_RS19705 0.56 -1.4 4,433,795 - BPHYT_RS19705 0.56 +2.3 4,433,795 - BPHYT_RS19705 0.56 -1.5 4,433,795 - BPHYT_RS19705 0.56 -0.5 4,433,795 - -0.5 4,434,036 + BPHYT_RS19705 0.81 -3.2 4,434,167 - -0.8 4,434,213 - +1.0 4,434,213 - -0.4 4,434,217 - -0.5 4,434,217 - -0.6 4,434,217 - -0.1 4,434,366 + +0.3 4,434,366 + -0.1 4,434,367 - -0.4 4,434,394 + -0.6 4,434,394 + +0.1 4,434,394 + -0.3 4,434,395 - +0.2 4,434,395 - -0.8 4,434,409 - -0.2 4,434,462 + +0.3 4,434,462 + +1.0 4,434,463 - +3.0 4,434,463 - -0.5 4,434,543 + -1.8 4,434,543 + +0.2 4,434,544 - -0.4 4,434,573 - BPHYT_RS19710 0.11 +1.3
Or see this region's nucleotide sequence