Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16520 and BPHYT_RS16525 are separated by 16 nucleotides BPHYT_RS16525 and BPHYT_RS16530 are separated by 111 nucleotides BPHYT_RS16530 and BPHYT_RS16535 are separated by 163 nucleotides
BPHYT_RS16520: BPHYT_RS16520 - major facilitator superfamily protein, at 3,745,052 to 3,746,218
_RS16520
BPHYT_RS16525: BPHYT_RS16525 - extradiol dioxygenase, at 3,746,235 to 3,746,633
_RS16525
BPHYT_RS16530: BPHYT_RS16530 - LysR family transcriptional regulator, at 3,746,745 to 3,747,728
_RS16530
BPHYT_RS16535: BPHYT_RS16535 - tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase, at 3,747,892 to 3,749,265
_RS16535
Position (kb)
3746
3747
3748 Strain fitness (log2 ratio)
-2
-1
0
1 at 3745.947 kb on + strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3745.973 kb on - strand, within BPHYT_RS16520 at 3746.237 kb on - strand at 3747.255 kb on + strand, within BPHYT_RS16530 at 3747.255 kb on + strand, within BPHYT_RS16530 at 3747.266 kb on + strand, within BPHYT_RS16530 at 3747.381 kb on + strand, within BPHYT_RS16530 at 3747.382 kb on - strand, within BPHYT_RS16530 at 3747.382 kb on - strand, within BPHYT_RS16530 at 3747.724 kb on + strand at 3747.726 kb on + strand at 3747.727 kb on - strand at 3747.727 kb on - strand at 3747.729 kb on + strand at 3747.737 kb on + strand at 3747.738 kb on - strand at 3747.797 kb on + strand at 3747.797 kb on + strand at 3747.798 kb on - strand at 3747.940 kb on + strand at 3747.940 kb on + strand at 3747.940 kb on + strand at 3747.966 kb on - strand at 3748.369 kb on + strand, within BPHYT_RS16535 at 3748.369 kb on + strand, within BPHYT_RS16535 at 3748.379 kb on - strand, within BPHYT_RS16535 at 3748.379 kb on - strand, within BPHYT_RS16535 at 3748.568 kb on - strand, within BPHYT_RS16535 at 3748.603 kb on + strand, within BPHYT_RS16535 at 3748.651 kb on + strand, within BPHYT_RS16535 at 3748.652 kb on - strand, within BPHYT_RS16535 at 3748.652 kb on - strand, within BPHYT_RS16535 at 3748.652 kb on - strand, within BPHYT_RS16535
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,745,947 + BPHYT_RS16520 0.77 +0.7 3,745,973 - BPHYT_RS16520 0.79 +0.5 3,745,973 - BPHYT_RS16520 0.79 -0.3 3,745,973 - BPHYT_RS16520 0.79 +0.0 3,746,237 - -0.1 3,747,255 + BPHYT_RS16530 0.52 +0.1 3,747,255 + BPHYT_RS16530 0.52 -0.1 3,747,266 + BPHYT_RS16530 0.53 +0.2 3,747,381 + BPHYT_RS16530 0.65 +0.3 3,747,382 - BPHYT_RS16530 0.65 +0.3 3,747,382 - BPHYT_RS16530 0.65 +1.5 3,747,724 + -0.0 3,747,726 + +0.7 3,747,727 - -0.8 3,747,727 - -0.2 3,747,729 + -0.2 3,747,737 + +0.5 3,747,738 - -0.5 3,747,797 + -0.0 3,747,797 + -0.5 3,747,798 - -2.3 3,747,940 + -0.1 3,747,940 + -1.3 3,747,940 + +0.3 3,747,966 - -0.7 3,748,369 + BPHYT_RS16535 0.35 +0.2 3,748,369 + BPHYT_RS16535 0.35 -0.5 3,748,379 - BPHYT_RS16535 0.35 -1.2 3,748,379 - BPHYT_RS16535 0.35 -0.5 3,748,568 - BPHYT_RS16535 0.49 +0.7 3,748,603 + BPHYT_RS16535 0.52 -2.5 3,748,651 + BPHYT_RS16535 0.55 -1.8 3,748,652 - BPHYT_RS16535 0.55 -0.5 3,748,652 - BPHYT_RS16535 0.55 +1.3 3,748,652 - BPHYT_RS16535 0.55 +0.5
Or see this region's nucleotide sequence