Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15510 and BPHYT_RS15515 are separated by 136 nucleotides BPHYT_RS15515 and BPHYT_RS15520 overlap by 1 nucleotides BPHYT_RS15520 and BPHYT_RS15525 overlap by 4 nucleotides BPHYT_RS15525 and BPHYT_RS15530 are separated by 7 nucleotides
BPHYT_RS15510: BPHYT_RS15510 - pyruvate dehydrogenase, at 3,518,512 to 3,520,236
_RS15510
BPHYT_RS15515: BPHYT_RS15515 - D-aminopeptidase, at 3,520,373 to 3,521,206
_RS15515
BPHYT_RS15520: BPHYT_RS15520 - D-aminopeptidase, at 3,521,206 to 3,522,306
_RS15520
BPHYT_RS15525: BPHYT_RS15525 - glutathione ABC transporter permease GsiD, at 3,522,303 to 3,523,208
_RS15525
BPHYT_RS15530: BPHYT_RS15530 - glutathione ABC transporter permease, at 3,523,216 to 3,524,136
_RS15530
Position (kb)
3521
3522
3523 Strain fitness (log2 ratio)
-2
-1
0
1 at 3520.212 kb on - strand at 3520.794 kb on + strand, within BPHYT_RS15515 at 3520.794 kb on + strand, within BPHYT_RS15515 at 3520.862 kb on - strand, within BPHYT_RS15515 at 3521.224 kb on + strand at 3521.225 kb on - strand at 3521.423 kb on + strand, within BPHYT_RS15520 at 3521.656 kb on - strand, within BPHYT_RS15520 at 3521.688 kb on + strand, within BPHYT_RS15520 at 3521.732 kb on + strand, within BPHYT_RS15520 at 3521.733 kb on - strand, within BPHYT_RS15520 at 3521.733 kb on - strand, within BPHYT_RS15520 at 3521.736 kb on + strand, within BPHYT_RS15520 at 3521.737 kb on - strand, within BPHYT_RS15520 at 3521.855 kb on + strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.856 kb on - strand, within BPHYT_RS15520 at 3521.909 kb on + strand, within BPHYT_RS15520 at 3522.545 kb on - strand, within BPHYT_RS15525 at 3522.633 kb on - strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.634 kb on + strand, within BPHYT_RS15525 at 3522.635 kb on - strand, within BPHYT_RS15525 at 3522.832 kb on + strand, within BPHYT_RS15525 at 3522.835 kb on + strand, within BPHYT_RS15525 at 3522.836 kb on - strand, within BPHYT_RS15525 at 3523.250 kb on + strand at 3523.250 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,520,212 - +0.8 3,520,794 + BPHYT_RS15515 0.50 +0.3 3,520,794 + BPHYT_RS15515 0.50 -0.6 3,520,862 - BPHYT_RS15515 0.59 -1.2 3,521,224 + +0.4 3,521,225 - -1.5 3,521,423 + BPHYT_RS15520 0.20 -2.0 3,521,656 - BPHYT_RS15520 0.41 +1.5 3,521,688 + BPHYT_RS15520 0.44 -0.3 3,521,732 + BPHYT_RS15520 0.48 -0.1 3,521,733 - BPHYT_RS15520 0.48 +0.6 3,521,733 - BPHYT_RS15520 0.48 -0.9 3,521,736 + BPHYT_RS15520 0.48 -0.9 3,521,737 - BPHYT_RS15520 0.48 -2.1 3,521,855 + BPHYT_RS15520 0.59 +1.5 3,521,856 - BPHYT_RS15520 0.59 +0.1 3,521,856 - BPHYT_RS15520 0.59 -0.4 3,521,856 - BPHYT_RS15520 0.59 -1.5 3,521,909 + BPHYT_RS15520 0.64 +0.2 3,522,545 - BPHYT_RS15525 0.27 +0.7 3,522,633 - BPHYT_RS15525 0.36 -0.6 3,522,634 + BPHYT_RS15525 0.37 -0.6 3,522,634 + BPHYT_RS15525 0.37 -0.2 3,522,634 + BPHYT_RS15525 0.37 -0.4 3,522,634 + BPHYT_RS15525 0.37 -0.9 3,522,634 + BPHYT_RS15525 0.37 +0.5 3,522,635 - BPHYT_RS15525 0.37 -0.1 3,522,832 + BPHYT_RS15525 0.58 -1.0 3,522,835 + BPHYT_RS15525 0.59 +0.4 3,522,836 - BPHYT_RS15525 0.59 -0.1 3,523,250 + +0.1 3,523,250 + -0.6
Or see this region's nucleotide sequence