Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14805 and BPHYT_RS14810 are separated by 238 nucleotides BPHYT_RS14810 and BPHYT_RS14815 are separated by 33 nucleotides BPHYT_RS14815 and BPHYT_RS14820 overlap by 11 nucleotides
BPHYT_RS14805: BPHYT_RS14805 - electron transfer flavoprotein subunit beta, at 3,369,106 to 3,369,855
_RS14805
BPHYT_RS14810: BPHYT_RS14810 - dioxygenase, at 3,370,094 to 3,370,891
_RS14810
BPHYT_RS14815: BPHYT_RS14815 - methionine ABC transporter permease, at 3,370,925 to 3,371,578
_RS14815
BPHYT_RS14820: BPHYT_RS14820 - methionine ABC transporter ATP-binding protein, at 3,371,568 to 3,372,602
_RS14820
Position (kb)
3370
3371 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3369.899 kb on - strand at 3369.901 kb on - strand at 3369.901 kb on - strand at 3369.938 kb on + strand at 3369.938 kb on + strand at 3369.938 kb on + strand at 3369.939 kb on - strand at 3369.939 kb on - strand at 3369.939 kb on - strand at 3370.039 kb on - strand at 3370.039 kb on - strand at 3370.039 kb on - strand at 3370.066 kb on + strand at 3370.082 kb on + strand at 3370.082 kb on + strand at 3370.083 kb on - strand at 3370.083 kb on - strand at 3370.095 kb on + strand at 3370.162 kb on - strand at 3370.193 kb on - strand, within BPHYT_RS14810 at 3370.193 kb on - strand, within BPHYT_RS14810 at 3370.230 kb on + strand, within BPHYT_RS14810 at 3370.230 kb on + strand, within BPHYT_RS14810 at 3370.554 kb on + strand, within BPHYT_RS14810 at 3370.555 kb on - strand, within BPHYT_RS14810 at 3370.632 kb on + strand, within BPHYT_RS14810 at 3370.633 kb on - strand, within BPHYT_RS14810 at 3370.698 kb on + strand, within BPHYT_RS14810 at 3370.699 kb on - strand, within BPHYT_RS14810 at 3370.699 kb on - strand, within BPHYT_RS14810 at 3370.909 kb on + strand at 3370.926 kb on + strand at 3370.927 kb on - strand at 3370.980 kb on - strand at 3370.980 kb on - strand at 3370.980 kb on - strand at 3371.044 kb on - strand, within BPHYT_RS14815 at 3371.287 kb on + strand, within BPHYT_RS14815 at 3371.287 kb on + strand, within BPHYT_RS14815 at 3371.463 kb on + strand, within BPHYT_RS14815 at 3371.463 kb on + strand, within BPHYT_RS14815 at 3371.464 kb on - strand, within BPHYT_RS14815 at 3371.578 kb on + strand at 3371.578 kb on + strand at 3371.578 kb on + strand at 3371.617 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,369,899 - -1.2 3,369,901 - +0.1 3,369,901 - -0.3 3,369,938 + +0.7 3,369,938 + +0.9 3,369,938 + +0.3 3,369,939 - -0.4 3,369,939 - +0.0 3,369,939 - +0.6 3,370,039 - +0.0 3,370,039 - +0.0 3,370,039 - -0.9 3,370,066 + -1.1 3,370,082 + -1.0 3,370,082 + +0.3 3,370,083 - +0.6 3,370,083 - +1.5 3,370,095 + +1.3 3,370,162 - -0.3 3,370,193 - BPHYT_RS14810 0.12 +0.5 3,370,193 - BPHYT_RS14810 0.12 -0.3 3,370,230 + BPHYT_RS14810 0.17 -1.0 3,370,230 + BPHYT_RS14810 0.17 +1.3 3,370,554 + BPHYT_RS14810 0.58 +0.2 3,370,555 - BPHYT_RS14810 0.58 -0.1 3,370,632 + BPHYT_RS14810 0.67 +0.4 3,370,633 - BPHYT_RS14810 0.68 +0.4 3,370,698 + BPHYT_RS14810 0.76 +2.3 3,370,699 - BPHYT_RS14810 0.76 -0.2 3,370,699 - BPHYT_RS14810 0.76 +1.6 3,370,909 + -0.1 3,370,926 + -1.3 3,370,927 - -0.5 3,370,980 - -0.1 3,370,980 - +1.0 3,370,980 - +1.0 3,371,044 - BPHYT_RS14815 0.18 -0.5 3,371,287 + BPHYT_RS14815 0.55 +0.3 3,371,287 + BPHYT_RS14815 0.55 -0.1 3,371,463 + BPHYT_RS14815 0.82 -1.2 3,371,463 + BPHYT_RS14815 0.82 +0.7 3,371,464 - BPHYT_RS14815 0.82 +1.2 3,371,578 + +3.3 3,371,578 + +0.2 3,371,578 + +0.2 3,371,617 + -0.9
Or see this region's nucleotide sequence