Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13900 and BPHYT_RS13905 are separated by 103 nucleotides BPHYT_RS13905 and BPHYT_RS13910 are separated by 64 nucleotides BPHYT_RS13910 and BPHYT_RS13915 overlap by 4 nucleotides
BPHYT_RS13900: BPHYT_RS13900 - ATPase, at 3,173,082 to 3,174,239
_RS13900
BPHYT_RS13905: BPHYT_RS13905 - ABC transporter ATP-binding protein, at 3,174,343 to 3,175,119
_RS13905
BPHYT_RS13910: BPHYT_RS13910 - 5-dehydro-2-deoxygluconokinase (EC 2.7.1.92); possible 5-dehydro-2-deoxyphosphogluconate aldolase DUF2090 (EC 4.1.2.29) (from data) , at 3,175,184 to 3,177,229
_RS13910
BPHYT_RS13915: BPHYT_RS13915 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, at 3,177,226 to 3,179,199
_RS13915
Position (kb)
3175
3176
3177
3178 Strain fitness (log2 ratio)
-1
0
1
2 at 3174.211 kb on - strand at 3175.545 kb on + strand, within BPHYT_RS13910 at 3175.631 kb on + strand, within BPHYT_RS13910 at 3175.631 kb on + strand, within BPHYT_RS13910 at 3175.968 kb on + strand, within BPHYT_RS13910 at 3176.654 kb on - strand, within BPHYT_RS13910 at 3176.847 kb on - strand, within BPHYT_RS13910 at 3177.240 kb on - strand at 3177.683 kb on + strand, within BPHYT_RS13915 at 3178.024 kb on + strand, within BPHYT_RS13915
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,174,211 - -0.8 3,175,545 + BPHYT_RS13910 0.18 +0.8 3,175,631 + BPHYT_RS13910 0.22 -0.0 3,175,631 + BPHYT_RS13910 0.22 -1.5 3,175,968 + BPHYT_RS13910 0.38 +1.3 3,176,654 - BPHYT_RS13910 0.72 +2.1 3,176,847 - BPHYT_RS13910 0.81 +0.1 3,177,240 - -0.7 3,177,683 + BPHYT_RS13915 0.23 +1.8 3,178,024 + BPHYT_RS13915 0.40 -0.3
Or see this region's nucleotide sequence