Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS13480

Experiment: 24hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotidesBPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotidesBPHYT_RS13485 and BPHYT_RS13490 are separated by 360 nucleotides BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011 _RS13475 BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853 _RS13480 BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364 _RS13485 BPHYT_RS13490: BPHYT_RS13490 - hypothetical protein, at 3,078,725 to 3,079,135 _RS13490 Position (kb) 3077 3078Strain fitness (log2 ratio) -3 -2 -1 0 1at 3076.249 kb on - strand, within BPHYT_RS13475at 3076.508 kb on + strand, within BPHYT_RS13475at 3077.094 kb on + strandat 3077.136 kb on - strandat 3077.183 kb on + strandat 3077.929 kb on - strandat 3077.937 kb on + strandat 3077.964 kb on + strandat 3077.964 kb on + strandat 3078.241 kb on - strandat 3078.654 kb on - strandat 3078.676 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1
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3,076,249 - BPHYT_RS13475 0.52 +0.0
3,076,508 + BPHYT_RS13475 0.68 -0.7
3,077,094 + -2.8
3,077,136 - +0.8
3,077,183 + -0.2
3,077,929 - +1.5
3,077,937 + +0.8
3,077,964 + -0.2
3,077,964 + +0.4
3,078,241 - +1.0
3,078,654 - +0.1
3,078,676 + +0.2

Or see this region's nucleotide sequence