Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13465 and BPHYT_RS13470 are separated by 94 nucleotides BPHYT_RS13470 and BPHYT_RS13475 are separated by 155 nucleotides BPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotides BPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotides
BPHYT_RS13465: BPHYT_RS13465 - hypothetical protein, at 3,074,444 to 3,074,824
_RS13465
BPHYT_RS13470: BPHYT_RS13470 - arylsulfate sulfotransferase, at 3,074,919 to 3,075,269
_RS13470
BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011
_RS13475
BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853
_RS13480
BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364
_RS13485
Position (kb)
3075
3076
3077
3078 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3074.563 kb on - strand, within BPHYT_RS13465 at 3074.631 kb on + strand, within BPHYT_RS13465 at 3074.632 kb on - strand, within BPHYT_RS13465 at 3074.765 kb on - strand, within BPHYT_RS13465 at 3074.839 kb on + strand at 3075.022 kb on + strand, within BPHYT_RS13470 at 3075.257 kb on - strand at 3075.259 kb on - strand at 3075.350 kb on + strand at 3075.688 kb on - strand, within BPHYT_RS13475 at 3075.861 kb on + strand, within BPHYT_RS13475 at 3075.867 kb on + strand, within BPHYT_RS13475 at 3075.868 kb on - strand, within BPHYT_RS13475 at 3076.093 kb on + strand, within BPHYT_RS13475 at 3076.249 kb on - strand, within BPHYT_RS13475 at 3076.508 kb on + strand, within BPHYT_RS13475 at 3077.094 kb on + strand at 3077.136 kb on - strand at 3077.183 kb on + strand at 3077.929 kb on - strand at 3077.937 kb on + strand at 3077.964 kb on + strand at 3077.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 3,074,563 - BPHYT_RS13465 0.31 +0.2 3,074,631 + BPHYT_RS13465 0.49 -1.7 3,074,632 - BPHYT_RS13465 0.49 -0.3 3,074,765 - BPHYT_RS13465 0.84 +0.1 3,074,839 + -0.1 3,075,022 + BPHYT_RS13470 0.29 +0.3 3,075,257 - -0.2 3,075,259 - -0.7 3,075,350 + +0.0 3,075,688 - BPHYT_RS13475 0.17 -0.6 3,075,861 + BPHYT_RS13475 0.27 -0.4 3,075,867 + BPHYT_RS13475 0.28 -0.7 3,075,868 - BPHYT_RS13475 0.28 -0.4 3,076,093 + BPHYT_RS13475 0.42 +0.0 3,076,249 - BPHYT_RS13475 0.52 +0.0 3,076,508 + BPHYT_RS13475 0.68 -0.7 3,077,094 + -2.8 3,077,136 - +0.8 3,077,183 + -0.2 3,077,929 - +1.5 3,077,937 + +0.8 3,077,964 + -0.2 3,077,964 + +0.4
Or see this region's nucleotide sequence