Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11530 and BPHYT_RS11535 are separated by 338 nucleotides BPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotides BPHYT_RS11540 and BPHYT_RS11545 overlap by 4 nucleotides
BPHYT_RS11530: BPHYT_RS11530 - 5-hydroxyisourate hydrolase, at 2,621,977 to 2,622,330
_RS11530
BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862
_RS11535
BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810
_RS11540
BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820
_RS11545
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.741 kb on - strand at 2621.799 kb on + strand at 2621.799 kb on + strand at 2621.800 kb on - strand at 2621.844 kb on - strand at 2621.885 kb on - strand at 2621.925 kb on - strand at 2622.274 kb on + strand, within BPHYT_RS11530 at 2622.282 kb on - strand, within BPHYT_RS11530 at 2622.307 kb on + strand at 2622.308 kb on - strand at 2622.627 kb on - strand at 2622.681 kb on - strand at 2622.993 kb on + strand, within BPHYT_RS11535 at 2623.081 kb on - strand, within BPHYT_RS11535 at 2623.143 kb on + strand, within BPHYT_RS11535 at 2623.422 kb on + strand, within BPHYT_RS11535 at 2623.753 kb on - strand at 2623.860 kb on + strand at 2623.861 kb on - strand at 2623.985 kb on - strand at 2624.020 kb on - strand at 2624.080 kb on + strand at 2624.081 kb on - strand at 2624.127 kb on + strand at 2624.129 kb on + strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.212 kb on + strand at 2624.363 kb on + strand, within BPHYT_RS11540 at 2624.400 kb on - strand, within BPHYT_RS11540 at 2624.539 kb on + strand, within BPHYT_RS11540 at 2624.540 kb on - strand, within BPHYT_RS11540
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 2,621,689 + +0.1 2,621,689 + -1.1 2,621,689 + -2.6 2,621,741 - -0.5 2,621,799 + -2.6 2,621,799 + -3.8 2,621,800 - +0.0 2,621,844 - +0.4 2,621,885 - -0.2 2,621,925 - +0.0 2,622,274 + BPHYT_RS11530 0.84 -0.4 2,622,282 - BPHYT_RS11530 0.86 +0.8 2,622,307 + -0.6 2,622,308 - -1.3 2,622,627 - +0.4 2,622,681 - +0.2 2,622,993 + BPHYT_RS11535 0.27 +0.0 2,623,081 - BPHYT_RS11535 0.35 -0.4 2,623,143 + BPHYT_RS11535 0.40 +0.9 2,623,422 + BPHYT_RS11535 0.63 +0.4 2,623,753 - +0.6 2,623,860 + -1.9 2,623,861 - -0.1 2,623,985 - -1.5 2,624,020 - -0.1 2,624,080 + +1.2 2,624,081 - +0.8 2,624,127 + -0.7 2,624,129 + +0.4 2,624,130 - +1.1 2,624,130 - -1.2 2,624,130 - +0.5 2,624,212 + +0.8 2,624,363 + BPHYT_RS11540 0.13 -0.7 2,624,400 - BPHYT_RS11540 0.20 -0.7 2,624,539 + BPHYT_RS11540 0.47 -1.5 2,624,540 - BPHYT_RS11540 0.47 -1.5
Or see this region's nucleotide sequence