Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11520 and BPHYT_RS11525 are separated by 13 nucleotides BPHYT_RS11525 and BPHYT_RS11530 are separated by 340 nucleotides
BPHYT_RS11520: BPHYT_RS11520 - hydroxydechloroatrazine ethylaminohydrolase, at 2,620,043 to 2,621,440
_RS11520
BPHYT_RS11525: BPHYT_RS11525 - hypothetical protein, at 2,621,454 to 2,621,636
_RS11525
BPHYT_RS11530: BPHYT_RS11530 - 5-hydroxyisourate hydrolase, at 2,621,977 to 2,622,330
_RS11530
Position (kb)
2621
2622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2620.607 kb on + strand, within BPHYT_RS11520 at 2620.608 kb on - strand, within BPHYT_RS11520 at 2620.676 kb on - strand, within BPHYT_RS11520 at 2621.014 kb on - strand, within BPHYT_RS11520 at 2621.018 kb on - strand, within BPHYT_RS11520 at 2621.019 kb on + strand, within BPHYT_RS11520 at 2621.019 kb on + strand, within BPHYT_RS11520 at 2621.019 kb on + strand, within BPHYT_RS11520 at 2621.019 kb on + strand, within BPHYT_RS11520 at 2621.019 kb on + strand, within BPHYT_RS11520 at 2621.020 kb on - strand, within BPHYT_RS11520 at 2621.020 kb on - strand, within BPHYT_RS11520 at 2621.020 kb on - strand, within BPHYT_RS11520 at 2621.020 kb on - strand, within BPHYT_RS11520 at 2621.193 kb on + strand, within BPHYT_RS11520 at 2621.193 kb on + strand, within BPHYT_RS11520 at 2621.319 kb on + strand at 2621.320 kb on - strand at 2621.621 kb on - strand at 2621.639 kb on + strand at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.741 kb on - strand at 2621.799 kb on + strand at 2621.799 kb on + strand at 2621.800 kb on - strand at 2621.844 kb on - strand at 2621.885 kb on - strand at 2621.925 kb on - strand at 2622.274 kb on + strand, within BPHYT_RS11530 at 2622.282 kb on - strand, within BPHYT_RS11530 at 2622.307 kb on + strand at 2622.308 kb on - strand at 2622.627 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 2,620,607 + BPHYT_RS11520 0.40 -0.3 2,620,608 - BPHYT_RS11520 0.40 -1.2 2,620,676 - BPHYT_RS11520 0.45 -0.1 2,621,014 - BPHYT_RS11520 0.69 -0.1 2,621,018 - BPHYT_RS11520 0.70 -0.3 2,621,019 + BPHYT_RS11520 0.70 +0.0 2,621,019 + BPHYT_RS11520 0.70 -0.5 2,621,019 + BPHYT_RS11520 0.70 -1.0 2,621,019 + BPHYT_RS11520 0.70 -1.7 2,621,019 + BPHYT_RS11520 0.70 +0.3 2,621,020 - BPHYT_RS11520 0.70 +0.6 2,621,020 - BPHYT_RS11520 0.70 +0.5 2,621,020 - BPHYT_RS11520 0.70 -1.0 2,621,020 - BPHYT_RS11520 0.70 +0.3 2,621,193 + BPHYT_RS11520 0.82 -1.3 2,621,193 + BPHYT_RS11520 0.82 +1.4 2,621,319 + -1.0 2,621,320 - +0.8 2,621,621 - +1.1 2,621,639 + -1.6 2,621,689 + +0.1 2,621,689 + -1.1 2,621,689 + -2.6 2,621,741 - -0.5 2,621,799 + -2.6 2,621,799 + -3.8 2,621,800 - +0.0 2,621,844 - +0.4 2,621,885 - -0.2 2,621,925 - +0.0 2,622,274 + BPHYT_RS11530 0.84 -0.4 2,622,282 - BPHYT_RS11530 0.86 +0.8 2,622,307 + -0.6 2,622,308 - -1.3 2,622,627 - +0.4
Or see this region's nucleotide sequence