Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09215 and BPHYT_RS09220 are separated by 40 nucleotides BPHYT_RS09220 and BPHYT_RS09225 are separated by 39 nucleotides BPHYT_RS09225 and BPHYT_RS09230 are separated by 177 nucleotides
BPHYT_RS09215: BPHYT_RS09215 - ATP-dependent DNA ligase, at 2,085,683 to 2,088,505
_RS09215
BPHYT_RS09220: BPHYT_RS09220 - hypothetical protein, at 2,088,546 to 2,088,851
_RS09220
BPHYT_RS09225: BPHYT_RS09225 - LysR family transcriptional regulator, at 2,088,891 to 2,089,832
_RS09225
BPHYT_RS09230: BPHYT_RS09230 - NADPH:quinone reductase, at 2,090,010 to 2,091,032
_RS09230
Position (kb)
2088
2089
2090 Strain fitness (log2 ratio)
-1
0
1 at 2088.009 kb on + strand, within BPHYT_RS09215 at 2088.010 kb on - strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.184 kb on + strand, within BPHYT_RS09215 at 2088.185 kb on - strand, within BPHYT_RS09215 at 2088.207 kb on - strand, within BPHYT_RS09215 at 2088.504 kb on - strand at 2088.541 kb on - strand at 2088.914 kb on - strand at 2088.914 kb on - strand at 2088.992 kb on - strand, within BPHYT_RS09225 at 2089.028 kb on - strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.066 kb on + strand, within BPHYT_RS09225 at 2089.067 kb on - strand, within BPHYT_RS09225 at 2089.516 kb on - strand at 2089.741 kb on - strand at 2089.911 kb on + strand at 2089.911 kb on + strand at 2090.010 kb on - strand at 2090.010 kb on - strand at 2090.034 kb on + strand at 2090.035 kb on - strand at 2090.216 kb on + strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230 at 2090.275 kb on - strand, within BPHYT_RS09230
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 2,088,009 + BPHYT_RS09215 0.82 -0.7 2,088,010 - BPHYT_RS09215 0.82 +0.2 2,088,184 + BPHYT_RS09215 0.89 +0.2 2,088,184 + BPHYT_RS09215 0.89 +0.8 2,088,184 + BPHYT_RS09215 0.89 +0.8 2,088,185 - BPHYT_RS09215 0.89 -0.4 2,088,207 - BPHYT_RS09215 0.89 +0.3 2,088,504 - +0.5 2,088,541 - -0.9 2,088,914 - +0.2 2,088,914 - +0.0 2,088,992 - BPHYT_RS09225 0.11 -1.7 2,089,028 - BPHYT_RS09225 0.15 -1.5 2,089,066 + BPHYT_RS09225 0.19 +1.4 2,089,066 + BPHYT_RS09225 0.19 -1.0 2,089,067 - BPHYT_RS09225 0.19 -0.9 2,089,516 - +0.4 2,089,741 - -0.0 2,089,911 + +0.4 2,089,911 + +0.2 2,090,010 - -0.1 2,090,010 - -0.1 2,090,034 + -0.6 2,090,035 - -1.0 2,090,216 + BPHYT_RS09230 0.20 -0.5 2,090,275 - BPHYT_RS09230 0.26 +0.2 2,090,275 - BPHYT_RS09230 0.26 -1.7
Or see this region's nucleotide sequence