Experiment: 24hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08700 and BPHYT_RS08705 are separated by 143 nucleotides BPHYT_RS08705 and BPHYT_RS08710 are separated by 430 nucleotides BPHYT_RS08710 and BPHYT_RS08715 overlap by 4 nucleotides BPHYT_RS08715 and BPHYT_RS08720 are separated by 150 nucleotides
BPHYT_RS08700: BPHYT_RS08700 - tRNA-dihydrouridine synthase A, at 1,975,614 to 1,976,615
_RS08700
BPHYT_RS08705: BPHYT_RS08705 - hypothetical protein, at 1,976,759 to 1,977,064
_RS08705
BPHYT_RS08710: BPHYT_RS08710 - hypothetical protein, at 1,977,495 to 1,979,087
_RS08710
BPHYT_RS08715: BPHYT_RS08715 - adenylyl-sulfate kinase, at 1,979,084 to 1,979,623
_RS08715
BPHYT_RS08720: BPHYT_RS08720 - membrane protein, at 1,979,774 to 1,980,400
_RS08720
Position (kb)
1977
1978
1979
1980 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1976.532 kb on + strand at 1976.685 kb on - strand at 1976.953 kb on + strand, within BPHYT_RS08705 at 1976.953 kb on + strand, within BPHYT_RS08705 at 1976.954 kb on - strand, within BPHYT_RS08705 at 1976.980 kb on + strand, within BPHYT_RS08705 at 1977.011 kb on - strand, within BPHYT_RS08705 at 1977.047 kb on - strand at 1977.059 kb on - strand at 1977.064 kb on + strand at 1977.065 kb on - strand at 1977.070 kb on + strand at 1977.314 kb on + strand at 1977.314 kb on + strand at 1977.314 kb on + strand at 1977.373 kb on - strand at 1977.392 kb on - strand at 1977.483 kb on - strand at 1977.483 kb on - strand at 1977.937 kb on - strand, within BPHYT_RS08710 at 1978.153 kb on - strand, within BPHYT_RS08710 at 1978.468 kb on - strand, within BPHYT_RS08710 at 1978.566 kb on + strand, within BPHYT_RS08710 at 1978.567 kb on - strand, within BPHYT_RS08710 at 1978.701 kb on + strand, within BPHYT_RS08710 at 1978.701 kb on + strand, within BPHYT_RS08710 at 1978.702 kb on - strand, within BPHYT_RS08710 at 1978.725 kb on + strand, within BPHYT_RS08710 at 1978.728 kb on + strand, within BPHYT_RS08710 at 1978.737 kb on + strand, within BPHYT_RS08710 at 1978.801 kb on - strand, within BPHYT_RS08710 at 1978.947 kb on + strand at 1978.948 kb on - strand at 1979.422 kb on - strand, within BPHYT_RS08715 at 1979.457 kb on - strand, within BPHYT_RS08715 at 1980.020 kb on - strand, within BPHYT_RS08720
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr PHYTO2 MOI 1 remove 1,976,532 + +0.2 1,976,685 - -1.9 1,976,953 + BPHYT_RS08705 0.63 -0.5 1,976,953 + BPHYT_RS08705 0.63 +0.5 1,976,954 - BPHYT_RS08705 0.64 +0.0 1,976,980 + BPHYT_RS08705 0.72 -0.2 1,977,011 - BPHYT_RS08705 0.82 +0.8 1,977,047 - -1.2 1,977,059 - -0.7 1,977,064 + -0.0 1,977,065 - -0.8 1,977,070 + -1.0 1,977,314 + -0.4 1,977,314 + +0.0 1,977,314 + +1.8 1,977,373 - +0.5 1,977,392 - -0.9 1,977,483 - +1.0 1,977,483 - +2.3 1,977,937 - BPHYT_RS08710 0.28 -0.9 1,978,153 - BPHYT_RS08710 0.41 -1.0 1,978,468 - BPHYT_RS08710 0.61 +0.0 1,978,566 + BPHYT_RS08710 0.67 +1.3 1,978,567 - BPHYT_RS08710 0.67 -1.5 1,978,701 + BPHYT_RS08710 0.76 -0.5 1,978,701 + BPHYT_RS08710 0.76 +0.0 1,978,702 - BPHYT_RS08710 0.76 -1.2 1,978,725 + BPHYT_RS08710 0.77 -2.9 1,978,728 + BPHYT_RS08710 0.77 -1.6 1,978,737 + BPHYT_RS08710 0.78 -0.7 1,978,801 - BPHYT_RS08710 0.82 -1.8 1,978,947 + -0.9 1,978,948 - +0.8 1,979,422 - BPHYT_RS08715 0.63 -0.7 1,979,457 - BPHYT_RS08715 0.69 -0.9 1,980,020 - BPHYT_RS08720 0.39 -0.0
Or see this region's nucleotide sequence